Identification and Detection of Botryosphaeria dothidea from Kiwifruit (Actinidia chinensis) in China

文献类型： 外文期刊

第一作者： Wang, Li

作者： Wang, Li;Hou, Hui;Zhou, Zengqiang;Tu, Hongtao;Yuan, Hongbo

作者机构：

关键词： kiwifruit ripe rot; Botryosphaeria dothidea; identification; LAMP; detection

期刊名称：PLANTS-BASEL （ 影响因子：3.935； ）

ISSN：

年卷期： 2021 年 10 卷 2 期

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收录情况： SCI

摘要： Kiwifruit is very popular among consumers due to its high nutritional value. The increasing expansion in kiwifruit cultivation has led to the spread of rot diseases. To identify the pathogens causing kiwifruit ripe rots in China, 24 isolates were isolated from the diseased fruit and wart in trees. Botryosphaeria dothidea was recognized as the pathogen causing kiwifruit ripe rot and wart in the tree through internal transcribed spacer (ITS) sequencing, pathogenicity testing, morphological and microscopic characteristics. The rapid and accurate detection of this pathogen will lead to better disease monitoring and control efforts. A loop-mediated isothermal amplification (LAMP) method was then developed to rapidly and specifically identify B. dothidea. These results offer value to further research into kiwifruit ripe rot, such as disease prediction, pathogen rapid detection, and effective disease control.

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