文献类型： 外文期刊
第一作者： Yuan, Hongbo
作者： Yuan, Hongbo;Hou, Hui;Huang, Tianxiang;Zhou, Zengqiang;Tu, Hongtao;Wang, Li
作者机构：
关键词： Pomegranate fruit rot; Coniella granati; Agrobacterium tumefaciens-mediated; transformation; GFP; Hyg
期刊名称：JOURNAL OF MICROBIOLOGICAL METHODS （ 影响因子：2.363； 五年影响因子：2.24 ）
ISSN： 0167-7012
年卷期： 2021 年 182 卷
页码：
收录情况： SCI
摘要： Pomegranate fruit rot caused by Coniella granati is among the most devastating diseases threatening pomegranate production. The pathogenic mechanism of this pathogen remains largely unknown due to lack of genetic transformation method. Herein, we developed an approach to the Agrobacterium tumefaciens-mediated transformation (ATMT) of C. granati using a plasmid vector encoding the green fluorescent protein (GFP) and hygromycin resistance (Hyg) genes. This approach yielded C. granati transformants that exhibited uniform, stable green fluorescence. We further optimized this ATMT protocol, enabling us to achieve a transformation efficiency of up to 300 transformants per 0.5 cm(2) mycelial plug. Together, we thus provide the first report of the stable transformation of C. granati, laying a foundation for future functional studies characterizing this economically important fungal pathogen.
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