Identification of gene co-expression networks and key genes regulating flavonoid accumulation in apple ( Malus x domestica ) fruit skin

文献类型： 外文期刊

第一作者： Ding, Tiyu

作者： Ding, Tiyu;Zhang, Ruiping;Zhang, Hengtao;Zhou, Zhe;Liu, Chonghuai;Wu, Mengmeng;Wang, Huan;Dong, Haiqing;Yao, Jia-Long;Yan, Zhenli;Yao, Jia-Long;Yan, Zhenli;Ding, Tiyu;Liu, Jihong

作者机构：

关键词： Flavonoid biosynthesis; Malus x domestica; Hub gene; Gene function identification; RNA-seq

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2021 年 304 卷

页码：

收录情况： SCI

摘要： Anthocyanin provides a red color for apple and health benefit for human. To better understand the molecular mechanisms of regulating apple color formation, we analyzed 27 transcriptomes of fruit skin from three cultivars 'Huashuo' (red-skinned), 'Hongcuibao' (red-skinned), and 'Golden Delicious' (yellow-skinned) at 0, 2, and 6 days after bag removal. Using pairwise comparisons and weighted gene co-expression network analyses (WGCNA), we constructed 17 co-expression modules. Among them, a specific module was negatively correlated to anthocyanin accumulation. The genes in the module are enriched in flavonoid biosynthesis pathways. These pathway genes were used to construct gene co-expression network of anthocyanin accumulation. Finally, a R2R3-MYB repressor designated MdMYB28 was identified as a key hub gene in the anthocyanin metabolism network. During the anthocyanin accumulation of apple fruit skin reaching a peak, MdMYB28 expression level was negatively correlated with the anthocyanin content. MdMYB28 was shown to directly bind to the promoter of MdMYB10 in yeast one-hybrid analyses. Over-expression of MdMYB28 decreased the anthocyanin biosynthesis in tobacco flower petals, suggesting that MdMYB28 acts as a negatively regulator of anthocyanin biosynthesis.

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