Genome-wide association studies of plant architecture-related traits and 100-seed weight in soybean landraces

文献类型： 外文期刊

第一作者： Zhang, Xiaoli

作者： Zhang, Xiaoli;Ding, Wentao;Xue, Dong;Li, Xiangnan;Zhou, Yang;Shen, Jiacheng;Feng, Jianying;Guo, Na;Xing, Han;Zhao, Jinming;Qiu, Lijuan

作者机构：

关键词： Soybean (Glycine max (L; ) Merr; ); Plant architecture-related traits; 100-seed weight; GWAS; Candidate genes

期刊名称：BMC GENOMIC DATA

ISSN：

年卷期： 2021 年 22 卷 1 期

页码：

收录情况： SCI

摘要： Background Plant architecture-related traits (e.g., plant height (PH), number of nodes on main stem (NN), branch number (BN) and stem diameter (DI)) and 100-seed weight (100-SW) are important agronomic traits and are closely related to soybean yield. However, the genetic basis and breeding potential of these important agronomic traits remain largely ambiguous in soybean (Glycine max (L.) Merr.). Results In this study, we collected 133 soybean landraces from China, phenotyped them in two years at two locations for the above five traits and conducted a genome-wide association study (GWAS) using 82,187 single nucleotide polymorphisms (SNPs). As a result, we found that a total of 59 SNPs were repeatedly detected in at least two environments. There were 12, 12, 4, 4 and 27 SNPs associated with PH, NN, BN, DI and 100-SW, respectively. Among these markers, seven SNPs (AX-90380587, AX-90406013, AX-90387160, AX-90317160, AX-90449770, AX-90460927 and AX-90520043) were large-effect markers for PH, NN, BN, DI and 100-SW, and 15 potential candidate genes were predicted to be in linkage disequilibrium (LD) decay distance or LD block. In addition, real-time quantitative PCR (qRT-PCR) analysis was performed on four 100-SW potential candidate genes, three of them showed significantly different expression levels between the extreme materials at the seed development stage. Therefore, Glyma.05 g127900, Glyma.05 g128000 and Glyma.05 g129000 were considered as candidate genes with 100-SW in soybean. Conclusions These findings shed light on the genetic basis of plant architecture-related traits and 100-SW in soybean, and candidate genes could be used for further positional cloning.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序