Cold tolerance SNPs and candidate gene mining in the soybean germination stage based on genome-wide association analysis

文献类型： 外文期刊

第一作者： Chen, Yuehan

作者： Chen, Yuehan;Ma, Qian;Chen, Yuehan;Liu, Zhi;Yang, Qing;Li, Chenhui;Shi, Xiaolei;Zhang, Mengchen;Yang, Chunyan;Yan, Long;Han, Dezhi;Jia, Hongchang;Wang, Shu;Lu, Wencheng;Qiu, Lijuan

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：4.4； 五年影响因子：5.0 ）

ISSN： 0040-5752

年卷期： 2024 年 137 卷 8 期

页码：

收录情况： SCI

摘要： Key messageThree QTLs associated with low-temperature tolerance were identified by genome-wide association analysis, and 15 candidate genes were identified by haplotype analysis and gene expression analyses.AbstractLow temperature is a critical factor affecting the geographical distribution, growth, development, and yield of soybeans, with cold stress during seed germination leading to substantial productivity loss. In this study, an association panel comprising 260 soybean accessions was evaluated for four germination traits and four cold tolerance index traits, revealing extensive variation in cold tolerance. Genome-wide association study (GWAS) identified 10 quantitative trait nucleotides (QTNs) associated with cold tolerance, utilizing 30,799 single nucleotide polymorphisms (SNPs) and four GWAS models. Linkage disequilibrium (LD) analysis positioned these QTNs within three cold-tolerance quantitative trait loci (QTL) and, with QTL19-1, was positioned by three multi-locus models, underscoring its importance as a key QTL. Integrative haplotype analysis, supplemented by transcriptome analysis, uncovered 15 candidate genes. The haplotypes within the genes Glyma.18G044200, Glyma.18G044300, Glyma.18G044900, Glyma.18G045100, Glyma.19G222500, and Glyma.19G222600 exhibited significant phenotypic variations, with differential expression in materials with varying cold tolerance. The QTNs and candidate genes identified in this study offer substantial potential for marker-assisted selection and gene editing in breeding cold-tolerant soybeans, providing valuable insights into the genetic mechanisms underlying cold tolerance during soybean germination.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序