Integrating Transcriptomics and Metabolomics to Characterize Metabolic Regulation to Elevated CO2 in Chlamydomonas Reinhardtii

文献类型： 外文期刊

第一作者： Zhang, Yufei

作者： Zhang, Yufei;Gu, Zipeng;Ren, Yudong;Wang, Lu;Zhang, Jian;Liang, Chengwei;Tong, Shanying;Wang, Yitao;Xu, Dong;Zhang, Xiaowen;Ye, Naihao;Wang, Yitao;Xu, Dong;Zhang, Xiaowen;Ye, Naihao

作者机构：

关键词： Chlamydomonas Reinhardtii; Elevated CO2; Long-term Acidification; Metabolomics; Transcriptomics

期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：3.619； 五年影响因子：3.322 ）

ISSN： 1436-2228

年卷期： 2021 年 23 卷 2 期

页码：

收录情况： SCI

摘要： With atmospheric CO2 increasing, a large amount of CO2 is absorbed by oceans and lakes, which changes the carbonate system and affects the survival of aquatic plants, especially microalgae. The main aim of our study was to explore the responses of Chlamydomonas reinhardtii (Chlorophyceae) to elevated CO2 by combined transcriptome and metabolome analysis under three different scenarios: control (CK, 400 ppm), short-term elevated CO2 (ST, 1000 ppm), and long-term elevated CO2 (LT, 1000 ppm). The transcriptomic data showed moderate changes between ST and CK. However, metabolic analysis indicated that fatty acids (FAs) and partial amino acids (AAs) were increased under ST. There was a global downregulation of genes involved in photosynthesis, glycolysis, lipid metabolism, and nitrogen metabolism but increase in the TCA cycle and beta-oxidation under LT. Integrated transcriptome and metabolome analyses demonstrated that the nutritional constituents (FAs, AAs) under LT were poor compared with CK, and most genes and metabolites involved in C and N metabolism were significantly downregulated. However, the growth and photosynthesis of cells under LT increased significantly. Thus, C. reinhardtii could form a specific adaptive evolution to elevated CO2, affecting future biogeochemical cycles.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序