Positive regulation of Type III secretion effectors and virulence by RyhB paralogs in Salmonella enterica serovar Enteritidis

文献类型： 外文期刊

第一作者： Chen, Binjie

作者： Chen, Binjie;Meng, Xianchen;Ni, Jie;He, Mengping;Chen, Yanfei;Xia, Pengpeng;Wang, Heng;Zhu, Guoqiang;Meng, Xia;Chen, Binjie;Meng, Xianchen;Ni, Jie;He, Mengping;Chen, Yanfei;Xia, Pengpeng;Wang, Heng;Zhu, Guoqiang;Meng, Xia;Liu, Siguo

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关键词： RyhB; Regulation of virulence; SipA; Invasion; Pathogenicity; Salmonella

期刊名称：VETERINARY RESEARCH （ 影响因子：3.683； 五年影响因子：4.106 ）

ISSN： 0928-4249

年卷期： 2021 年 52 卷 1 期

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收录情况： SCI

摘要： Small non-coding RNA RyhB is a key regulator of iron homeostasis in bacteria by sensing iron availability in the environment. Although RyhB is known to influence bacterial virulence by interacting with iron metabolism related regulators, its interaction with virulence genes, especially the Type III secretion system (T3SS), has not been reported. Here, we demonstrate that two RyhB paralogs of Salmonella enterica serovar Enteritidis upregulate Type III secretion system (T3SS) effectors, and consequently affect Salmonella invasion into intestinal epithelial cells. Specifically, we found that RyhB-1 modulate Salmonella response to stress condition of iron deficiency and hypoxia, and stress in simulated intestinal environment (SIE). Under SIE culture conditions, both RyhB-1 and RyhB-2 are drastically induced and directly upregulate the expression of T3SS effector gene sipA by interacting with its 5 ' untranslated region (5 ' UTR) via an incomplete base-pairing mechanism. In addition, the RyhB paralogs upregulate the expression of T3SS effector gene sopE. By regulating the invasion-related genes, RyhBs in turn affect the ability of S. Enteritidis to adhere to and invade into intestinal epithelial cells. Our findings provide evidence that RyhBs function as critical virulence factors by directly regulating virulence-related gene expression. Thus, inhibition of RyhBs may be a potential strategy to attenuate Salmonella.

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