Binding affinity-guided design of a highly sensitive noncompetitive immunoassay for small molecule detection

文献类型： 外文期刊

第一作者： Bai, Yuchen

作者： Bai, Yuchen;Wang, Yahui;Li, Qiang;Dou, Leina;Liu, Minggang;Shao, Shibei;Zhu, Jianyu;Shen, Jianzhong;Wang, Zhanhui;Wen, Kai;Yu, Wenbo;Wang, Yahui

作者机构：

关键词： Small molecules; Noncompetitive immunoassay; Affinity; Halofuginone

期刊名称：FOOD CHEMISTRY （ 影响因子：6.306； 五年影响因子：6.219 ）

ISSN： 0308-8146

年卷期： 2021 年 351 卷

页码：

收录情况： SCI

摘要： Small molecules are immunochemically classified as hapten that lacking of at least two epitopes, usually using competitive format for establishing immunoassays. However, theoretically, noncompetitive immunoassay format is more sensitive and has a wider analytical range. In the present study, a novel hapten of halofuginone was synthesized and used to produce a monoclonal antibody (mAb). By analyzing the binding kinetics, we found that the affinity of analyte?enzyme to mAb was much greater than that of analyte, which could result in a low sensitivity of competitive assay format. Based on this, we established a novel noncompetitive immunoassay by using a replacement approach. The noncompetitive format has obvious advantages in sensitivity and analytical range, which promoted approximately 3.5? and 5?fold, respectively, compared to the competitive immunoassay. Ultimately, the newly designed noncompetitive immunoassay in this work will provide insights as well as alternative method to traditional small molecule competitive assays.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序