Characteristics of Brucella abortus vaccine strain A19 reveals its potential mechanism of attenuated virulence

文献类型： 外文期刊

第一作者： Cheng, Zhimin

作者： Cheng, Zhimin;Li, Zichen;Yin, Yi;Lian, Zhengmin;Abdelgawad, Hosny Ahmed;Hu, Hai;Guan, Xiang;Zuo, Dong;Ding, Chan;Wang, Shaohui;Li, Tao;Qi, Jingjing;Tian, Mingxing;Yu, Shengqing;Cheng, Zhimin;Cai, Yumei

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关键词： Brucella abortus; Vaccine strain A19; Phenotypic analysis; Characteristics; Virulence

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.03； 五年影响因子：2.923 ）

ISSN： 0378-1135

年卷期： 2021 年 254 卷

页码：

收录情况： SCI

摘要： Brucella vaccination is one of the most important strategies for controlling brucellosis in livestock. The A19 strain was the effective vaccine used to control brucellosis in China. However, the characteristics of physiological and attenuated virulence of the A19 strain are not investigated in detail. In this study, we compared the phenotypic characteristics of the A19 to the wild-type strain S2308. Virulence test showed that the A19 was significantly attenuated at chronic infection stage in infected mouse model. In growth analysis, the A19 exhibited a quick growth at exponential phase and premature at stationary phase. The inflammatory response of macrophages infected by the A19 was detected using TaqMan qPCR assay, indicating that the inflammatory level of the A19-infected macrophages was higher than that of the S2308 infection. Cell death analysis showed that the A19 was not cytotoxic for macrophages. Cell infection showed that the A19 reduced its ability to invade, survive and traffic within host cells, and the intracellular A19 hardly excludes lysosome-associated marker LAMP-1, suggesting that the A19 can't escape the lysosome degradation within host cells. In further study, the sensitivity test exhibited that the A19 is more sensitive to stress and bactericidal factors than the S2308 strain, Western blot and silver staining analysis exhibited that the A19 has a different expression pattern of OMPs and reduces LPS O antigen expression relative to the S2308 strain. Those data give us a more detailed understanding about the A19 vaccine strain, which will be beneficial for improvement of current Brucella vaccine and overcoming its defects.

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