A ratiometric fluorescent probe for monitoring the changes in the level of hydrogen polysulfides in mitochondria during stimulus-induced redox imbalance

文献类型： 外文期刊

第一作者： Han, Qingxin

作者： Han, Qingxin;Yang, Lingna;Zhang, Huijie;Jiang, Huie;Wang, Xuechuan;Ru, Jiaxi;Song, Yunyun

作者机构：

关键词： Hydrogen polysulfides; Mitochondria-targetable; Fluorescent probe; Redox imbalance; Ratiometric

期刊名称：DYES AND PIGMENTS （ 影响因子：4.613； 五年影响因子：3.881 ）

ISSN： 0143-7208

年卷期： 2021 年 188 卷

页码：

收录情况： SCI

摘要： Hydrogen polysulfides (H2Sn, n > 1) are crucial for physiological functions and pathological processes. They are highly desirable for developing mitochondria-targetable fluorescent probes to monitor the fluctuation of H2Sn level when the intracellular redox state changes. Here, we report a ratiometric fluorescent probe Mito-RHP for detecting H2Sn in mitochondria by integrating the triphenylphosphonium and 2-fluoro-5-nitrobenzoic ester groups with a 1,8-naphthalimide-based fluorophore. High sensitivity and selectivity to endogenous and exogenous H2Sn was observed. Mito-RHP was stable under normal conditions and was an efficient fluorescent probe for bioimaging. Bioimaging experiments demonstrated that Mito-RHP possessed a good mitochondria-targeting property and showed a ratiometric response to H2Sn. Further, it could be used for H2Sn detection in living cells under stimulus-induced redox imbalance. These advantages enable us to further understand H2Sn-related physiological and pathological effects.

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