Identification and evaluation of midgut protein RL12 of Dermacentor silvarum interacting with Anaplasma ovis VirD4

文献类型： 外文期刊

第一作者： Iqbal, Naveed

作者： Iqbal, Naveed;Mukhtar, Muhammad Uzair;Yang, Jifei;Niu, Qingli;Li, Zhi;Zhao, Shuaiyang;Zhao, Yaru;Guan, Guiquan;Liu, Zhijie;Yin, Hong;Yin, Hong

作者机构：

关键词： Anaplasma ovis; Dermacentor silvarum; Yeast two-hybrid; GST pull-down; Midgut; Pathogen-vector interaction

期刊名称：TICKS AND TICK-BORNE DISEASES （ 影响因子：2.749； 五年影响因子：2.896 ）

ISSN： 1877-959X

年卷期： 2021 年 12 卷 3 期

页码：

收录情况： SCI

摘要： Anaplasma ovis, a tick-borne intra-erythrocytic Gram-negative bacterium, is a causative agent of ovine anaplasmosis. It is known that Dermacentor ticks act as biological vectors for A. ovis. VirD4 is the machine component of Type IV Secretion System of A. ovis. To better understand the pathogen-vector interaction, VirD4 was used as a bait protein for screening midgut proteins of Dermacentor silvarum via yeast two-hybrid mating assay. As a result, a ribosomal protein RL12 was identified from the midgut cDNA library of D. silvarum. For further validation, using in vitro Glutathione S-transferase (GST) pull-down assay, interaction between the proteins, GST-RL12 and HIS-VirD4, was observed in Western blot analysis. The study is first of its kind reporting a D. silvarum midgut protein interaction with VirD4 from A. ovis. Functional annotations showed some important cellular processes are attributed to the protein, particularly in the stringent response and biogenesis. The results of the study suggest the involvement of the VirD4-RL12 interaction in the regulation of signaling pathways, which is a tool for understanding the pathogen-vector interaction.

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