Genome-Wide Identification and Analysis of Chitinase-Like Gene Family in Bemisia tabaci (Hemiptera: Aleyrodidae)
文献类型: 外文期刊
第一作者: Peng, Zhengke
作者: Peng, Zhengke;Ren, Jun;Zeng, Yang;Tian, Lixia;Wang, Shaoli;Wu, Qingjun;Xie, Wen;Zhang, Youjun;Peng, Zhengke;Liang, Pei;Su, Qi
作者机构:
关键词: Bemisia tabaci; chitinase; glycosyl hydrolase family; phylogenetic analysis; RNA interference
期刊名称:INSECTS ( 影响因子:2.22; )
ISSN:
年卷期: 2021 年 12 卷 3 期
页码:
收录情况: SCI
摘要: Simple Summary For the sake of growth and development, old exuviums of Bemisia tabaci nymphs should be periodically substituted by new ones until a final emergence turns them into adults. During ecdysis, chitinases are of great importance in chitin degradation and turnover of cuticles, which provides us potential targets for RNA interference-based B. tabaci management. Therefore, we annotated 14 chitinase and chitinase-like genes in B. tabaci based on data of genome and transcriptomes. With the help of a nanomaterial-promoted RNAi method, we found that silencing of BtCht10, BtCht5, and BtCht7 resulted in significant increase of death rate on B. tabaci nymphs and the developmental duration was noticeably postponed for BtCht2-silenced nymphs. Chitinases are of great importance in chitin degradation and remodeling in insects. However, the genome-wide distribution of chitinase-like gene family in Bemsia tabaci, a destructive pest worldwide, is still elusive. With the help of bioinformatics, we annotated 14 genes that encode putative chitinase-like proteins, including ten chitinases (Cht), three imaginal disk growth factors (IDGF), and one endo-beta-N-acetylglucosaminidase (ENGase) in the genome of the whitefly, B. tabaci. These genes were phylogenetically grouped into eight clades, among which 13 genes were classified in the glycoside hydrolase family 18 groups and one in the ENGase group. Afterwards, developmental expression analysis suggested that BtCht10, BtCht5, and BtCht7 were highly expressed in nymphal stages and exhibit similar expression patterns, implying their underlying role in nymph ecdysis. Notably, nymphs exhibited a lower rate of survival when challenged by dsRNA targeting these three genes via a nanomaterial-promoted RNAi method. In addition, silencing of BtCht10 significantly resulted in a longer duration of development compared to control nymphs. These results indicate a key role of BtCht10, BtCht5, and BtCht7 in B. tabaci nymph molting. Our research depicts the differences of chitinase-like family genes in structure and function and identified potential targets for RNAi-based whitefly management.
分类号:
- 相关文献
作者其他论文 更多>>
-
Removal of PAHs in paddy fields: How natural periphytic biofilm enhances the removal process and the mechanisms involved
作者:Li, Su-Xin;Ji, Fang-Ling;Jia, Ling-Yun;Ren, Jun;Gao, Xiao-Rong;Lv, Jun
关键词:Paddy field; Periphytic biofilm; Polycyclic aromatic hydrocarbons (PAHs); Adsorption; Biodegradation; Microbial community
-
Adsorption effect and mechanism of Cd(II) by different phosphorus-enriched biochars
作者:Zeng, Yang;Lin, Yuhan;Ma, Ming;Chen, Hong;Ma, Ming;Chen, Hong;Ma, Ming;Chen, Hong
关键词:Phosphorous-enriched biochar; Agricultural and forestry waste; Low-grade phosphate rock and derivatives; Cadmium; Adsorption mechanism; Mineral precipitation
-
A Maximum Dose Bioassay to Assess Efficacy of Spinetoram against Cowpea Thrip Megalurothrips usitatus in China
作者:Yu, Huailiang;Wu, Mingyue;Li, Shaoka;Zou, Xiang;Kong, Xiangyi;Yu, Huailiang;Li, Shaoka;Li, Jin;Guo, Zhaojiang;Wu, Qingjun;Zhang, Youjun;Xie, Wen;Guo, Zhaojiang;Wu, Qingjun;Zhang, Youjun;Xie, Wen
关键词:cowpea thrip; Megalurothrips usitatus (Bagnall); spinetoram; maximum dose bioassay
-
GPCR-MAPK signaling pathways underpin fitness trade- offs in whitefly
作者:Fu, Buli;Liang, Jinjin;Hu, Jinyu;Du, Tianhua;He, Chao;Wei, Xuegao;Gong, Peipan;Yang, Jing;Liu, Shaonan;Huang, Mingjiao;Yang, Xin;Zhang, Youjun;Fu, Buli;Hu, Jinyu;Liu, Kui;Du, Tianhua;Wei, Xuegao;Gui, Lianyou;Zhang, Youjun;Tan, Qimei;He, Chao;Liu, Shaonan;Huang, Mingjiao;Zhang, Youjun;Wei, Xuegao;Nauen, Ralf;Bass, Chris
关键词:adaptive evolution; fitness trade- offs; GPCR; MAPK; P450
-
Nutritional Status, Sex, and Ambient Temperature Modulate the Wingbeat Frequency of the Diamondback Moth Plutella xylostella
作者:Wang, Menglun;Wang, Menglun;Wang, Jialin;Liang, Pei;Wu, Kongming
关键词:Plutella xylostella; wingbeat frequency; stroboscope; temperature; humidity
-
Dual mutations in the whitefly nicotinic acetylcholine receptor β1 subunit confer target-site resistance to multiple neonicotinoid insecticides
作者:Yin, Cheng;Liu, Shao-Nan;Du, Tian-Hua;Gong, Pei-Pan;Wei, Xue-Gao;Yang, Jing;Huang, Ming-Jiao;Fu, Bu-Li;Liang, Jin-Jin;Xue, Hu;Hu, Jin-Yu;Ji, Yao;He, Chao;Du, He;Wang, Chao;Zhang, Rong;Lu, Han-Tang;Xie, Wen;Yang, Xin;Zhang, You-Jun;Yin, Cheng;Gui, Lian-You;OReilly, Andrias O.;Zhang, Cheng-Jia;Tan, Qi-Mei;Chu, Dong;Zhou, Xu-Guo;Nauen, Ralf;Bass, Chris
关键词:
-
Identification of an Entomopathogenic Fungus, Pseudozyma flocculosa (Traquair, Shaw & Jarvis), and Its Efficacy against Tetranychus urticae Koch
作者:Zhang, Yan;Dong, Rui;Hu, Shouyin;Guo, Zhaojiang;Wang, Shaoli
关键词:fungus identification; two-spotted spider mite; virulence activity; biological control