Both TGF-beta 1 and Smad4 regulate type I collagen expression in the muscle of grass carp, Ctenopharyngodon idella

文献类型： 外文期刊

第一作者： Xia, Yun

作者： Xia, Yun;Yu, Ermeng;Li, Zhifei;Zhang, Kai;Tian, Jingjing;Wang, Guangjun;Xie, Jun;Gong, Wangbao

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关键词： TGF-β 1; Smad4; Type I collagen; Grass carp; RNAi

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.242； 五年影响因子：2.326 ）

ISSN： 0920-1742

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收录情况： SCI

摘要： Type I collagen is proven to make an important contribution to fish muscle quality. Our previous study has shown the Smad4-dependent regulation of type I collagen expression in the muscle of crisp grass carp fed with faba bean. However, the regulatory roles of TGF-beta 1 or TGF-beta 1/Smad4 on type I collagen remain unclear in ordinary grass carp fed with normal diets or in other fish species. To clarify this point, the effect of TGF-beta 1 and Smad4 over-expression and RNAi knockdown on type I collagen (COL1-alpha 1 and COL1-alpha 2) expression were tested in vitro (zebrafish ZF4 cells) and in vivo (grass carp) along with the TGF-beta 1/Smad4 co-expression and co-knockdown. The mRNA levels of TGF-beta 1, Smad4, and type I collagen were upregulated in the groups with over-expressed TGF-beta 1 and Smad4 and downregulated in the groups of TGF-beta 1 and Smad4 RNAi in comparison to controls in vitro (P < 0.05). Similarly, in the in vivo experiment, the mRNA abundance of TGF-beta 1, Smad4, and type I collagen of over-expression group was higher than the controls at 36 h (P < 0.05). Co-injection of TGF-beta 1/Smad4 over-expression and RNAi vectors generally showed the higher efficacy. This study revealed that TGF-beta 1 and Smad4 genes regulated type I collagen expression in grass carp muscle and zebrafish. These findings will provide references for the collagen regulation of other freshwater fishes.

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