Transcriptome and plant hormone analyses provide new insight into the molecular regulatory networks underlying hybrid lethality in cabbage (Brassica oleracea)

文献类型： 外文期刊

第一作者： Xiao, Zhiliang

作者： Xiao, Zhiliang;Liu, Xing;Fang, Zhiyuan;Yang, Limei;Zhang, Yangyong;Wang, Yong;Zhuang, Mu;Honghao Lv

作者机构：

关键词： Defence response; Hybrid lethality; Plant hormone; Programmed cell death; Transcriptome analysis

期刊名称：PLANTA （ 影响因子：3.39； 五年影响因子：3.687 ）

ISSN： 0032-0935

年卷期： 2021 年 253 卷 5 期

页码：

收录情况： SCI

摘要： Main conclusion Comparative morphological, transcriptomic and phytohormone analyses reveal a defence network leading to PCD involved in cabbage hybrid lethality. Hybrid lethality (HL) plays an essential role in the stability of a population by blocking gene exchange between species, but the molecular mechanism remains largely undetermined. In this study, we performed phenotype, transcriptome and plant hormone analyses of HL in cabbage. Phenotype analysis confirmed that HL is characterised by a typical programmed cell death (PCD) process. A time-resolved RNA-Seq identified 2724 differentially expressed genes (DEGs), and functional annotations analyses revealed that HL was closely associated with the defence response. A defence regulation network was constructed based on the plant-pathogen interaction pathway and MAPK signalling pathway, which comprised DEGs related to Ca2+ and hydrogen peroxide (H2O2) leading to PCD. Moreover, important DEGs involved in hormone signal transduction pathways including salicylic acid (SA) and jasmonic acid (JA) were identified, which were further confirmed by endogenous and exogenous SA and JA measurements. Our results identified key genes and pathways in the regulating network of HL in cabbage, and might open the gate for revealing the molecular mechanism of HL in plants.

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