Reproductive performance is associated with seasonal plasma reproductive hormone levels, steroidogenic enzymes and sex hormone receptor expression levels in cultured Asian yellow pond turtles (Mauremys mutica)

文献类型： 外文期刊

第一作者： Wang, Yakun

作者： Wang, Yakun;Liu, Xiaoli;Li, Wei;Zhao, Jian;Liu, Haiyang;Yu, Lingyun;Zhu, Xinping

作者机构：

关键词： Fecundity; Seasonally breeding; Steroidogenic enzymes; Hormone receptor; Mauremys mutica

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.219； 五年影响因子：2.046 ）

ISSN： 1096-4959

年卷期： 2021 年 254 卷

页码：

收录情况： SCI

摘要： In order to understand the endocrine mechanism associated with fecundity of seasonally breeding animals, we investigated the plasma reproductive hormones levels and detected the differences in steroidogenic enzymes and sex hormone receptor mRNA levels in female Mauremys mutica. These turtles were divided into higher fecundity (HF) group than those in lower fecundity (LF) group based on paternity identification in our previous research. The plasma estrogen (E2), testosterone (T) and progesterone (P4) levels were significantly higher in pre-breeding season (PBS) than those in non-breeding season (NBS) and were markedly higher in the HF group than those in LF group. In the hypothalamus, there was significantly higher mRNA abundance of P450-cholesterol side-chain cleavage enzyme (P450Scc) encoded by Cyp11 alpha 1, aromatase (Cyp19 alpha 1) and 5-reductase (5 alpha-R), but significantly lower mRNA levels of follicular stimulating hormone receptor (FSHR) and progesterone receptor (PR) detected in PBS than those in NBS. The pituitary steroidogenic acute regulatory protein (StAR), cytochrome P450-17alpha-hydroxylase (Cyp17 alpha 1), 3-hydroxy-steroid dehydrogenase (3 beta HSD), 17-hydroxy-steroid dehydrogenase 3 (17 beta HSD3), Cyp19 alpha l, 5 alpha-R, FSHR, estrogen receptor 1 (ESR1), androgen receptor (AR) and PR transcriptional levels in HF group were up-regulated significantly compared with the LF group. In the ovary, Cyp17 alpha 1 and 17pHSD3 transcriptional levels were markedly higher in PBS than those in NBS. We detected significantly increased expression levels of all steroidogenic enzymes, but notably lower mRNA levels of FSHR and PR in uterus during the PBS, and the HF group has significantly higher expression levels of StAR, Cyp17 alpha 1, 5 alpha-R and AR than LF group. Our work reveals seasonal variations in hormone regulation as well as gene regulation in turtles, providing reliable information to understand the mechanisms underlying the different reproductive capacity of reptiles.

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