Expression and Polymorphism Analysis of CFL2 Gene in Chinese Dabieshan Cattle

文献类型： 外文期刊

第一作者： Zhao, Shuanping

作者： Zhao, Shuanping;Xu, Lei;Jin, Hai;Jia, Yutang

作者机构：

关键词： CFL2 gene; Dabieshan (DBS) cattle; Polymorphism detection; Expression; Association analysis

期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.79； 五年影响因子：0.713 ）

ISSN： 0030-9923

年卷期： 2021 年 53 卷 3 期

页码：

收录情况： SCI

摘要： Cofilin 2 (CFL2) is essential for skeletal muscle development and maintenance through regulating the length of actin filaments. In this study, we aimed to identify common variations in CFL2 gene and investigate their effects on growth traits in Chinese Dabieshan (DBS) cattle. By DNA sequencing and (forced) PCR-RFLP methods, three polymorphisms (g.1500G 0.5), and SNP g.2213C 0.25). LD (Linkage disequilibrium) analysis showed that SNP g.1694T A and g.2213C>G had a strong linkage (r2>0.33), a total of four different haplotypes were constructed and the frequency of the main haplotypes AAG accounted for over 61.2 % of the total individuals. Association analysis indicated that all of the three SNPs were significantly associated with growth traits in the detected population. Furthermore, real-time PCR indicated that CFL2 mRNA was varied expressed in all studied tissues. The results of our study provide evidence that polymorphisms in CFL2 gene are associated with growth traits, and CFL2 gene could be utilized as molecular markers for future assisted selection in cattle breeding program.

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