Molecular identification and functional analysis of MyD88 in giant freshwater prawn (Macrobrachium rosenbergii) and expression changes in response to bacterial challenge
文献类型： 外文期刊
第一作者： Gao, Quanxin
作者： Gao, Quanxin;Tang, Qiongying;Yi, Shaokui;Li, Jingfen;Xing, Qianqian;Luo, Jinping;Yang, Guoliang;Gao, Quanxin;Tang, Qiongying;Yi, Shaokui;Li, Jingfen;Xing, Qianqian;Luo, Jinping;Yang, Guoliang;Gao, Quanxin;Tang, Qiongying;Yi, Shaokui;Li, Jingfen;Xing, Qianqian;Luo, Jinping;Yang, Guoliang;Gao, Quanxin;Tang, Qiongying;Yi, Shaokui;Li, Jingfen;Xing, Qianqian;Luo, Jinping;Yang, Guoliang;Xia, Zhenglong;Cai, Miuying;Du, Houkuan;Yang, Jie;Yang, Guoliang
作者机构：
关键词： MyD88; Macrobrachium rosenbergii; Cloning
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：5.162； 五年影响因子：5.137 ）
ISSN： 0141-8130
年卷期： 2021 年 178 卷
页码：
收录情况： SCI
摘要： Myeloid differentiation factor 88 (MyD88) is a crucial adaptor protein for Toll-like receptor (TLR)-mediated signaling pathways and plays an important role in immune response. In this study, the full-length cDNA of MyD88 from Macrobrachium rosenbergii (MRMyD88) was cloned. The MRMyD88 cDNA is 1758 bp long and contains a 1398-bp open reading frame. Multiple sequence alignment and phylogenetic analysis revealed that the amino acid sequence of MRMyD88 shared high identity with the known MyD88 proteins. The MRMyD88 mRNA was widely expressed in all examined tissues, with highest level in intestine, followed by gonad and pleopod. Furthermore, the MRMyD88 promoter region, spanning 1622 bp, contains several transcription factor-binding sites, including nine GATA-1 box motifs. Electrophoretic mobility shift assay showed that Gfi-1, SRF, and Oct-1 bind to the upstream region of MRMyD88. Additionally, the results showed that the expression levels of TLR1, TLR2 and TLR3 were different in response to Vibrio anguillarum, Lactobacillus plantarum and Aeromonas hydrophila infections. However, these bacteria significantly increased the expression levels of MyD88 and prophenoloxidase. These data suggest that the TLR-mediated signaling pathway is MyD88-dependent in response to pathogenic and probiotic bacteria in M. rosenbergii. (c) 2021 Published by Elsevier B.V.
分类号：
- 相关文献
作者其他论文 更多>>
-
Whole-Genome Resequencing Analysis of the Camelus bactrianus (Bactrian Camel) Genome Identifies Mutations and Genes Affecting Milk Production Traits
作者：Yao, Huaibing;Zhao, Zhongkai;Yang, Jie;Yao, Huaibing;Zhao, Zhongkai;Yang, Jie;Pan, Zhangyuan;Ma, Wanpeng;Su, Zhanqiang
关键词：Bactrian camel; milk production traits; genomic analysis; SNP; association analysis
-
Coinfection with infectious precocity virus and Decapod iridescent virus 1 in farmed giant freshwater prawn ( Macrobrachium rosenbergii )
作者：Wang, Guohao;Guo, Xiaomeng;Zhou, Chengyan;Huang, Jie;Yao, Jiayun;Wang, Guohao;Guo, Xiaomeng;Zhou, Chengyan;Lou, Haoyu;Li, Xuan;Dong, Xuan;Huang, Jie;Li, Xuan;Dong, Xuan;Dong, Xuan;Yang, Guoliang;Huang, Jie;Huang, Jie;Yao, Jiayun;Dong, Xuan
关键词：IPV; DIV1; Coinfection; Macrobrachium rosenbergii
-
Identification and Transcriptome Analysis of a Novel Allelic Mutant of NAL1 in Rice
作者：Wang, Yang;Xu, Wanxin;Liu, Yan;Yang, Jie;Guo, Xin;Zhang, Jiaruo;Pu, Jisong;Zhang, Wenfeng;Wang, Yang;Zhang, Wenfeng;Chen, Nenggang
关键词：rice; narrow leaf allelic mutant; gene cloning; NAL1; RNA-Seq
-
Transcriptome analysis of the gonad reveals growth differences between large, medium and small individuals in a pure family of Macrobrachium rosenbergii
作者：Liu, Peimin;Zhao, Xiuxin;Tang, Qiongying;Li, Jingfen;Dong, Haiyan;Yang, Guoliang;Yi, Shaokui;Gao, Quanxin;Xia, Zhenglong;Yang, Guoliang;Yi, Shaokui;Gao, Quanxin
关键词：Macrobrachium Rosenbergii; Gonad; RNA -sequencing; Growth; Differentially expressed genes
-
A deep learning-based approach for fully automated segmentation and quantitative analysis of muscle fibers in pig skeletal muscle
作者：Yao, Zekai;Li, Hao;Li, Xinxin;Li, Jianhao;Luo, Yizhi;Meng, Fanming;Yao, Zekai;Zheng, Enqin;Yang, Jie;Li, Hao;Li, Xinxin;Wang, Ting;Fan, Zhenfei;Zhan, Yuexin;Yang, Yingshan;Wu, Zhenfang;Yao, Zekai;Zheng, Enqin;Yang, Jie;Li, Hao;Li, Xinxin;Wang, Ting;Fan, Zhenfei;Zhan, Yuexin;Yang, Yingshan;Wu, Zhenfang;Wo, Jingjie;Yin, Ling;Wu, Zhenfang;Luo, Yizhi;Zheng, Enqin;Yang, Jie;Wu, Zhenfang
关键词：Pigs; Skeletal muscle; Deep learning; Image segmentation; Quantitative analysis
-
Multi-omic characterization of allele-specific regulatory variation in hybrid pigs
作者：Quan, Jianping;Wang, Xingwang;Cai, Gengyuan;Ding, Rongrong;Zhuang, Zhanwei;Zhou, Shenping;Ruan, Donglin;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Meng, Fanming;Wu, Jie;Xu, Cineng;Qiu, Yibin;Wang, Shiyuan;Lin, Meng;Li, Shaoyun;Ye, Yong;Zhou, Fuchen;Lin, Danyang;Li, Xuehua;Deng, Shaoxiong;Zhang, Yuling;Yao, Zekai;Yang, Yingshan;Liu, Yiyi;Zhan, Yuexin;Zhang, Jiaming;Ma, Fucai;Yang, Jifei;Chen, Qiaoer;Yang, Jisheng;Gu, Ting;Huang, Sixiu;Xu, Zheng;Li, Zicong;Yang, Jie;Wu, Zhenfang;Quan, Jianping;Ding, Rongrong;Tan, Suxu;Huang, Wen;Quan, Jianping;Cai, Gengyuan;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Lin, Meng;Huang, Sixiu;Yang, Jie;Quan, Jianping;Ding, Rongrong;Ye, Jian;Dong, Linsong;Wu, Zhenfang;Yang, Ming;Gao, Xin;Liu, Zhihong;Yang, Ming;Ding, Rongrong;Ye, Jian;Dong, Linsong;Wu, Zhenfang;Wang, Xingwang;Cai, Gengyuan;Zhuang, Zhanwei;Zhou, Shenping;Ruan, Donglin;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Wu, Jie;Xu, Cineng;Qiu, Yibin;Wang, Shiyuan;Li, Shaoyun;Ye, Yong;Zhou, Fuchen;Lin, Danyang;Li, Xuehua;Deng, Shaoxiong;Zhang, Yuling;Yao, Zekai;Yang, Yingshan;Liu, Yiyi;Zhan, Yuexin;Zhang, Jiaming;Ma, Fucai;Yang, Jifei;Gu, Ting;Xu, Zheng;Li, Zicong;Yang, Jie;Meng, Fanming
关键词：
-
4D label-free quantitative proteomic analysis identifies CRABP1 as a novel candidate gene for litter size in rabbits
作者：Bao, Zhiyuan;Chen, Yang;Li, Jiali;Cai, Jiawei;Yang, Jie;Zhao, Bohao;Wu, Xinsheng;Chen, Yang;Zhao, Bohao;Wu, Xinsheng;Zhai, Pin
关键词：rabbits; ovary; proteome; litter size; CRABP1
