A colorimetric aptasensor for the simple and rapid detection of human papillomavirus type 16 L1 proteins

文献类型： 外文期刊

第一作者： Zhu, Li

作者： Zhu, Li;Zhao, Yu;Yao, Shangchen;Xu, Mingzhe;Yin, Lihui;Zhai, Xihai;Teng, Xu

作者机构：

期刊名称：ANALYST （ 影响因子：3.978； 五年影响因子：3.928 ）

ISSN： 0003-2654

年卷期： 2021 年 146 卷 8 期

页码：

收录情况： SCI

摘要： In this study, a novel colorimetric aptasensor was developed for the rapid detection and visual screening of HPV16 L1 proteins using gold nanoparticles (AuNPs) and an RNA aptamer against HPV16 L1 protein (APT(HPV16 L1)). The AuNP-APT(HPV16 L1) conjugates could be aggregated by the addition of a salt in the presence of HPV16 L1 proteins at the ppb level. At the same time, the surface plasma resonance absorption peaks of AuNPs shifted to a short wavelength, and an observable change in color from red to blue occurred. The relative absorbance (A(blank) - A(sample)/A(blank)) at 520 nm exhibited a stable response to HPV16 L1 proteins over a concentration range from 9.6 to 201.6 ng mL(-1). The visual detection limit of HPV16 L1 proteins was found to be 9.6 ng mL(-1). Finally, the proposed colorimetric aptasensor was successfully applied for the rapid and effective detection of HPV16 L1 proteins in clinical samples and vaccine samples. The validity and reliability of the proposed colorimetric aptasensor were verified by the enzyme-linked immunosorbent assay method. The proposed colorimetric aptasensor provided a promising indicator for screening and quantitative detection of HPV16 L1 proteins in clinical samples.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序