Development and application of Recombinase Polymerase Amplification assay for detection of Bipolaris sorokiniana
文献类型： 外文期刊
第一作者： Zhao, Wei
作者： Zhao, Wei;Chi, Yuan-kai;Wang, Tao;Xu, A-mei;Qi, Ren-de;Ye, Meng-di
作者机构：
关键词： Recombinase polymerase amplification; Bipolaris sorokiniana; Detection; Wheat
期刊名称：CROP PROTECTION （ 影响因子：2.381； 五年影响因子：2.537 ）
ISSN： 0261-2194
年卷期： 2021 年 145 卷
页码：
收录情况： SCI
摘要： Bipolaris sorokiniana is a fungal pathogen that caused root rot and spot blotch on wheat. A Recombinase Polymerase Amplification (RPA) assay was developed to detect B. sorokiniana specifically based on the sequences of calmodulin gene sequence. The specificity of RPA assay was tested against 19 fungal organisms associated with wheat, and the detection limit was determined to be 10 pg for B. sorokiniana pure fungal DNA. Further, the RPA assay was capable of detecting B. sorokiniana from artificially inoculated and field collected wheat tissues directly. These results indicate that the RPA assay is rapid and effective method for detection and molecular identification of B. sorokiniana.
分类号：
- 相关文献
作者其他论文 更多>>
-
Metagenomic profiling uncovers microbiota and antibiotic resistance patterns across human, chicken, pig fecal, and soil environments
作者：Bai, Xue;Wang, Tao;Li, Diyan;Bai, Xue;Li, Mingzhou;Zhong, Hang;Sun, Jing;Cui, Xiang;Gu, Yiren;Miao, Xiaomeng;Li, Jing;Lu, Lizhi;Xu, Wenwu;Sun, Jing
关键词：Metagenome; ARGs; Animal gut; Soil; Indicators
-
Genetic Variations of African Swine Fever Virus: Major Challenges and Prospects
作者：Chen, Shengmei;Fu, Qiang;Qiu, Hua-Ji;Chen, Shengmei;Wang, Tao;Luo, Rui;Lu, Zhanhao;Lan, Jing;Sun, Yuan;Qiu, Hua-Ji;Lan, Jing;Qiu, Hua-Ji
关键词：African swine fever; African swine fever virus; mutations; recombination; vaccines
-
Nutrient and metabolite characteristics of the husk, bran and millet isolated from the foxtail millet (Setaria italica L.) during polishing
作者：Li, Pengliang;Cai, Xinru;Li, Shaohui;Zhao, Wei;Liu, Junli;Zhang, Xiaodi;Zhang, Aixia;Liu, Jingke;Li, Zengning;Li, Zengning;Guo, Linlin
关键词：Foxtail millet; Husk; Bran; Nutrient; Metabolomics
-
Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
作者：Xu, Sheng-yang;Feng, Xiao-ran;Zhao, Wei;Bi, Yan-liang;Diao, Qi-yu;Tu, Yan
关键词：Gastrointestinal microbiome; Gastrointestinal metabolome; Host metabolome; Holstein heifer calves; Preweaning ADG
-
The effect of heat moisture treatment times on physicochemical and digestibility properties of adzuki bean, pea, and white kidney bean flours and starches
作者：Li, Shaohui;Li, Pengliang;Zhao, Wei;Zhang, Aixia;Liu, Jingke;Zhang, Liu;Sheng, Qinghai
关键词：Heat moisture treatment; Beans; Physicochemical properties; Digestibility
-
Antioxidant activity of Phellinus igniarius fermentation mycelia contributions of different solvent extractions and their inhibitory effect on α-amylase
作者：Dong, Yating;Wang, Tao;Gan, Bingcheng;Zhao, Jin;Duan, Xinlian;Cao, Luping;Feng, Rencai;Miao, Renyun;Yan, Junjie;Wu, Zhi;Dong, Yating;Wang, Tao;Gan, Bingcheng;Zhao, Jin;Duan, Xinlian;Cao, Luping;Feng, Rencai;Miao, Renyun;Yan, Junjie;Wu, Zhi;Wasser, Solomon P.;Wasser, Solomon P.;Zhang, Zhiyuan
关键词：Phellinus igniarius QB72; Fermentation mycelia; Polysaccharides; alpha-amylase inhibitory
-
Physicochemical properties of whole grain foxtail millet sourdough and steamed bread quality: Co-fermentation of lactic acid bacteria and yeast in whole grain fermented foods
作者：Li, Shaohui;Yi, Huaxi;Li, Shaohui;Zhang, Aixia;Zhao, Wei;Li, Pengliang;Si, Xingxue;Liu, Jingke
关键词：Sourdough; Physicochemical characterization; Flavor; Foxtail millet steamed bread
