Development and application of Recombinase Polymerase Amplification assay for detection of Bipolaris sorokiniana
文献类型： 外文期刊
第一作者： Zhao, Wei
作者： Zhao, Wei;Chi, Yuan-kai;Wang, Tao;Xu, A-mei;Qi, Ren-de;Ye, Meng-di
作者机构：
关键词： Recombinase polymerase amplification; Bipolaris sorokiniana; Detection; Wheat
期刊名称：CROP PROTECTION （ 影响因子：2.381； 五年影响因子：2.537 ）
ISSN： 0261-2194
年卷期： 2021 年 145 卷
页码：
收录情况： SCI
摘要： Bipolaris sorokiniana is a fungal pathogen that caused root rot and spot blotch on wheat. A Recombinase Polymerase Amplification (RPA) assay was developed to detect B. sorokiniana specifically based on the sequences of calmodulin gene sequence. The specificity of RPA assay was tested against 19 fungal organisms associated with wheat, and the detection limit was determined to be 10 pg for B. sorokiniana pure fungal DNA. Further, the RPA assay was capable of detecting B. sorokiniana from artificially inoculated and field collected wheat tissues directly. These results indicate that the RPA assay is rapid and effective method for detection and molecular identification of B. sorokiniana.
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