Development of a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay for the Detection of Haemonchus contortus in Goat Feces
文献类型： 外文期刊
第一作者： Wu, Yao-Dong
作者： Wu, Yao-Dong;Xu, Min-Jun;Wu, Yao-Dong;Wang, Qi-Qi;Wang, Meng;Zhu, Xing-Quan;Elsheikha, Hany M.;Yang, Xin;Hu, Min;Zhu, Xing-Quan
作者机构：
关键词： Haemonchus contortus; goat; recombinase polymerase amplification; lateral flow strip; rapid detection
期刊名称：KOREAN JOURNAL OF PARASITOLOGY （ 影响因子：1.311； 五年影响因子：1.395 ）
ISSN： 0023-4001
年卷期： 2021 年 59 卷 2 期
页码：
收录情况： SCI
摘要： Haemonchosis remains a significant problem in small ruminants. In this study, the assay of recombinase polymerase amplification (RPA) combined with the lateral flow strip (LFS-RPA) was established for the rapid detection of Haemonchus contortus in goat feces. The assay used primers and a probe targeting a specific sequence in the ITS-2 gene. We compared the performance of the LFS-RPA assay to a PCR assay. The LFS-RPA had a detection limit of 10 fg DNA, which was 10 times less compared to the lowest detection limit obtained by PCR. Out of 24 goat fecal samples, LFS-RPA assay detected H. contortus DNA with 95.8% sensitivity, compared to PCR, 79.1% sensitivity. LFS-RPA assay did not detect DNA from other related helminth species and demonstrated an adequate tolerance to inhibitors present in the goat feces. Taken together, our results suggest that LFS-RPA assay had a high diagnostic accuracy for the rapid detection of H. contortus and merits further evaluation.
分类号：
- 相关文献
作者其他论文 更多>>
-
Chemotaxis of rhizosphere Pseudomonas sp. induced by foliar spraying of lanthanum reduces cadmium uptake by pakchoi
作者：Wang, Meng;Yu, Lei;Wang, Jing;Qin, Luyao;Sun, Xiaoyi;Liu, Jiaxiao;Han, Yun;Chen, Shibao
关键词：Cadmium toxicity; Plant growth; Root exudates; Pseudomonas; Chemotaxis
-
Biodegradable film drip fertigation is more conducive to reducing the diversity and abundance of antibiotic resistance genes than plastic film drip fertigation
作者：Li, Yingjie;Yuan, Yuhan;Li, Cuilan;Zhang, Jinjing;Liu, Hang;Wang, Meng;Li, Qian;Zhang, Lei;Sun, Bo;Qin, Yubo
关键词：Antibiotic resistance genes; Biodegradable film drip fertigation; Bacterial diversity; HT-PCR
-
Exploring variances in meat quality between Qingyuan partridge chicken and Cobb broiler: Insights from combined multi-omics analysis
作者：Yang, Xin;Cai, Bolin;Zhang, Zhaofeng;Mo, Yu;Zhou, Zhen;Wu, Ruiquan;Kong, Shaofen;Cai, Danfeng;Zhang, Ruitong;Li, Zhenhui;Nie, Qinghua;Yang, Xin;Cai, Bolin;Zhang, Zhaofeng;Mo, Yu;Zhou, Zhen;Wu, Ruiquan;Kong, Shaofen;Cai, Danfeng;Li, Zhenhui;Nie, Qinghua
关键词：Qingyuan partridge chicken; Commercial broiler; Meat quality; Multi-omics analysis
-
Long short-term search session-based document re-ranking model
作者：Liu, Jianping;Wang, Meng;Wang, Yingfei;Chu, Xintao;Liu, Jianping;Wang, Jian
关键词：Document re-ranking; Long short-term session search; Memory network; BERT; User intent
-
Effects of the skp1 gene of the SCF complex on lipid metabolism and response to abiotic stress in Chlamydomonas reinhardtii
作者：Deng, Xiao Dong;Fei, Xiao Wen;Deng, Xiao Dong;Wang, Meng;Liu, Si Hang;Xu, Dian Long;Deng, Xiao Dong;Wang, Meng;Liu, Si Hang;Xu, Dian Long;Deng, Xiao Dong;Deng, Xiao Dong
关键词：SCF(SKP1-Cullin1-F-box) complex; Skp1; E3 ubiquitin ligase; Chlamydomonas reinhardtii; lipid metabolism; abiotic stress
-
The UDP-glycosyltransferase UGT352A3 contributes to the detoxification of thiamethoxam and imidacloprid in resistant whitefly
作者：Du, Tianhua;Xue, Hu;Zhang, Youjun;Yang, Xin;Du, Tianhua;Zhou, Xiaomao;Gui, Lianyou;Belyakova, Natalia A.
关键词：UDP-glycosyltransferases; Enzyme activity; Bemisia tabaci; Insecticide resistance; Molecular docking
-
Magnetic Co/Fe bimetallic confined nitrogen dopped porous carbon to activate peroxymonosulfate for efficient degradation of mycotoxins: Radical and non-radical pathways
作者：Wang, Yudan;Li, Yu;Zhai, Wenlei;Fang, Lin;Wang, Meng
关键词：Co/Fe@N-PC; Mycotoxins; Peroxymonosulfate; DFT calculations; Toxicity evaluation
