Development of a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay for the Detection of Haemonchus contortus in Goat Feces

文献类型： 外文期刊

第一作者： Wu, Yao-Dong

作者： Wu, Yao-Dong;Xu, Min-Jun;Wu, Yao-Dong;Wang, Qi-Qi;Wang, Meng;Zhu, Xing-Quan;Elsheikha, Hany M.;Yang, Xin;Hu, Min;Zhu, Xing-Quan

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关键词： Haemonchus contortus; goat; recombinase polymerase amplification; lateral flow strip; rapid detection

期刊名称：KOREAN JOURNAL OF PARASITOLOGY （ 影响因子：1.311； 五年影响因子：1.395 ）

ISSN： 0023-4001

年卷期： 2021 年 59 卷 2 期

页码：

收录情况： SCI

摘要： Haemonchosis remains a significant problem in small ruminants. In this study, the assay of recombinase polymerase amplification (RPA) combined with the lateral flow strip (LFS-RPA) was established for the rapid detection of Haemonchus contortus in goat feces. The assay used primers and a probe targeting a specific sequence in the ITS-2 gene. We compared the performance of the LFS-RPA assay to a PCR assay. The LFS-RPA had a detection limit of 10 fg DNA, which was 10 times less compared to the lowest detection limit obtained by PCR. Out of 24 goat fecal samples, LFS-RPA assay detected H. contortus DNA with 95.8% sensitivity, compared to PCR, 79.1% sensitivity. LFS-RPA assay did not detect DNA from other related helminth species and demonstrated an adequate tolerance to inhibitors present in the goat feces. Taken together, our results suggest that LFS-RPA assay had a high diagnostic accuracy for the rapid detection of H. contortus and merits further evaluation.

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