Genetic Mapping and Identification of the Candidate Gene for White Seed Coat in Cucurbita maxima

文献类型： 外文期刊

第一作者： Shi, Yuzi

作者： Shi, Yuzi;Zhang, Meng;Shu, Qin;Ma, Wei;Sun, Tingzhen;Xiang, Chenggang;Wang, Changlin;Duan, Ying

作者机构：

关键词： seed coat color; map-based cloning; phenylpropanoid; flavonoid metabolism pathway; Cucurbita maxima

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.556； 五年影响因子：4.653 ）

ISSN：

年卷期： 2021 年 22 卷 6 期

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收录情况： SCI

摘要： Seed coat color is an important agronomic trait of edible seed pumpkin in Cucurbita maxima. In this study, the development pattern of seed coat was detected in yellow and white seed coat accessions Wuminglv and Agol. Genetic analysis suggested that a single recessive gene white seed coat (wsc) is involved in seed coat color regulation in Cucurbita maxima. An F-2 segregating population including 2798 plants was used for fine mapping and a candidate region containing nine genes was identified. Analysis of 54 inbred accessions revealed four main Insertion/Deletion sites in the promoter of CmaCh15G005270 encoding an MYB transcription factor were co-segregated with the phenotype of seed coat color. RNA-seq analysis and qRT-PCR revealed that some genes involved in phenylpropanoid/flavonoid metabolism pathway displayed remarkable distinction in Wuminglv and Agol during the seed coat development. The flanking InDel marker S1548 was developed to predict the seed coat color in the MAS breeding with an accuracy of 100%. The results may provide valuable information for further studies in seed coat color formation and structure development in Cucurbitaceae crops and help the molecular breeding of Cucurbita maxima.

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