文献类型： 外文期刊
第一作者： Chen, Xi
作者： Chen, Xi;Yang, Jin-Xian;Li, Ying-Ying;Song, Tie-Ying;Ge, Jun-Qing
作者机构：
关键词： Anguillid herpesvirus; ORF95; Late gene; Subcellular localization; Envelope protein
期刊名称：VIRUS GENES （ 影响因子：1.991； 五年影响因子：1.776 ）
ISSN： 0920-8569
年卷期：
页码：
收录情况： SCI
摘要： Anguillid herpesvirus 1 (AngHV) is one of the vital pathogenic agents found in the wild and cultured eel populations, which has brought significant losses to eel culture industry in China. In this study, AngHV ORF95 was characterized. Bioinformatics analysis showed that ORF95 putatively encodes a structural protein that is homologous to hemagglutinin-esterase (HE) protein of infectious salmon anemia virus (ISAV). Temporal transcription and expression analysis indicated that ORF95 is a viral late gene. Subcellular localization analysis revealed that ORF95 was predominantly localized in the cytoplasm. Further, western blot analysis indicated that ORF95 is a structural protein of virion envelope. These results provide a novel basis to make further efforts to clarify the function of ORF95 in the process of AngHV infection and the possibility to use ORF95 as antigen to develop AngHV subunit vaccine.
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