Ectopic expression of GhIQD14 (cotton IQ67 domain-containing protein 14) causes twisted organ and modulates secondary wall formation in Arabidopsis

文献类型： 外文期刊

第一作者： Wang, Li

作者： Wang, Li;Liu, Yujie;Liu, Chen;Luo, Ming;Ge, Changwei;Xu, Fan

作者机构：

关键词： Cotton; IQD; Microtubule binding protein; Secondary wall formation; Twisted organ

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：3.72； 五年影响因子：3.966 ）

ISSN： 0981-9428

年卷期： 2021 年 163 卷

页码：

收录情况： SCI

摘要： In plants, although KNOX genes are known to regulate secondary cell wall (SCW) formation, their proteinregulating mechanisms remain largely unknown. Here, we showed that GhKNL1, which regulates SCW formation and fiber development in cotton, could interact with an IQ67 domain containing protein (GhIQD14) in yeast. Confocal observation showed that GhIQD14 was localized to the microtubules. In Arabidopsis, ectopic expression of GhIQD14 caused hypocotyls to be sensitive to microtubule depolymerization agent, organ twisting of seedlings, trichomes, rosette leaves, and capsules, as well as severely irregular xylem vessels and thicker interfascicular fiber cell walls in the inflorescence stem. Furthermore, we found that GhIQD14 interacted with AtKNAT7 in vivo, and instantaneous co-expression of GhIQD14 and AtKNAT7 in tobacco showed that GhIQD14 weakened the distribution of AtKNAT7 in the nucleus, bringing it into the microtubules, thus affecting the SCW formation related genes expression. Our results suggested that GhIQD14 might be involved in the morphological development and SCW formation in cotton.

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