Fumarylacetoacetate hydrolase is required for fertility in rice

文献类型： 外文期刊

第一作者： Hu, Chao

作者： Hu, Chao;Huang, Lihua;Ren, Chunmei;Huang, Lihua;Liu, Jinling;Wang, Zhilong;Ren, Chunmei;Chen, Yancheng;Gao, Bida;Zhu, Qi

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关键词： Mutant complementation; OsFAH; Oryza sativa L; Sterility; Tyrosine degradation pathway

期刊名称：PLANTA （ 影响因子：3.39； 五年影响因子：3.687 ）

ISSN： 0032-0935

年卷期： 2021 年 253 卷 6 期

页码：

收录情况： SCI

摘要： Main conclusion The riceOsFAH gene functions identically to that ofArabidopsis SSCD1 encoding FAH. Loss ofOsFAH causes rice sterility. Fumarylacetoacetate hydrolase (FAH) is the last enzyme in the tyrosine (Tyr) degradation pathway that is crucial for animals. By genetic analysis of the mutant of Short-day Sensitive Cell Death 1 gene encoding Arabidopsis FAH, we first found the pathway also plays a critical role in plants (Han et al., Plant Physiol 162:1956-1964, 2013). To further understand the role of the Tyr degradation pathway in plants, we investigated a biological function of the rice FAH. Firstly, the cDNA of rice FAH gene (OsFAH) was cloned and confirmed to be able to rescue the ArabidopsisShort-day Sensitive Cell Death 1 mutant defective in the FAH. Then, we identified the OsFAH T-DNA insertion mutant and generated the OsFAH RNA interference lines, and found that loss of OsFAH results in rice sterility. Furthermore, we analyzed expression of the OsFAH gene in roots, stems, leaves and young panicles at booting stage of rice and found that its transcript level was highest in young panicles and lowest in roots. In addition, the expression analysis of beta-glucuronidase driven by OsFAH promoter in transgenic Arabidopsis showed that the OsFAH promoter was highly active in aerial tissues in vegetative stage, and sepals, filaments and stigma in reproductive stage. These results suggested that FAH plays an important role in rice fertility.

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