Insight into the mechanisms of methane production enhancement by bioaugmentation with H2/CO2 in anaerobic digestion

文献类型： 外文期刊

第一作者： Yang, Jiancheng

作者： Yang, Jiancheng;Xing, Tao;Sun, Yongming;Li, Lianhua;Li, Ying;Guo, Ying;Zhen, Feng;Pang, Yuwan;Xing, Tao;Sun, Yongming;Li, Lianhua;Li, Ying;Guo, Ying;Zhen, Feng;Yang, Jiancheng;Qu, Bin;Yang, Haojun

作者机构：

关键词： Hydrogenotrophic methanogens; Molar ratios; Domestication; Microbial community

期刊名称：INTERNATIONAL JOURNAL OF HYDROGEN ENERGY （ 影响因子：8.3； 五年影响因子：7.7 ）

ISSN： 0360-3199

年卷期： 2025 年 113 卷

页码：

收录情况： SCI

摘要： In this study, the high performance hydrogenotrophic methanogenic bacteria were domesticated at different H2/ CO2 molar ratios, and the influence of the addition of high performance hydrogenotrophic methanogenic bacteria on the methanogenic performance of the anaerobic digestion (AD) system and the change of microbial community structure were investigated. 16s rDNA showed that Methanobrevibacter was the dominant methanogenic bacterium at different methane production experiment results showed H2/CO2 molar ratios, and the maximum cumulative methane production of 220.87 +/- 6.55 mL/g VS was obtained in the bioaugmentation experiments when using a domesticating agent with an H2/CO2 molar ratio of 4. In addition, the domesticating agent enhanced the hydrolysis and acidification steps, and changed the microbial community structure. This study demonstrated the potential of different H2/CO2 molar ratios for the domestication of high-performance hydrogen-based methanogenic bacteria.It would lay the foundation for the development of high-load anaerobic digestion in the future, and had great significance for the efficient and stable operation of biogas and biogas engineering.

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