Integrated slip valve-assisted fluidic chip coupling with CRISPR/Cas12a system for nucleic acid analysis

文献类型： 外文期刊

第一作者： Qian, Siwenjie

作者： Qian, Siwenjie;Chen, Yanju;Wu, Jian;Peng, Cheng;Wang, Xiaofu;Xu, Junfeng;Che, Yang;Wang, Tingzhang;Wu, Jian

作者机构：

关键词： Nucleic acid detection; Integrated chip; Dual detection; Loop -mediated isothermal amplification; CRISPR; Cas12a

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.2； 五年影响因子：5.9 ）

ISSN： 0003-2670

年卷期： 2023 年 1239 卷

页码：

收录情况： SCI

摘要： Currently, some on-site nucleic acid detection platforms have been developed. However, these platforms still need to be improved in device integration and multiple detection capability. In this work, an integrated dual nucleic acid analysis platform was developed by slip valve-assisted fluidic chip coupled with CRISPR/Cas12a system. All the reagents, including nucleic acid extraction, air-dried loop-mediated isothermal amplification (LAMP) and CRISPR/Cas12a detection reagents, were preloaded on the fluidic chip. Liquids transfer and stirring could be controlled by a slip valve and a syringe. By combining duplex LAMP reaction with two CRISPR detection units, CRISPR/Cas12a-based dual nucleic acid analysis was successfully constructed. Benefiting from highquality nucleic acid extraction on the chip, as low as 30 copies/reaction of Vibrio parahaemolyticus (V. parahaemolyticus) and 20 copies/reaction of Salmonella typhimurium (S. typhimurium) could be simultaneously detected. Detection results could be observed by the naked eye under a portable ultraviolet lamp. The whole

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序