Transcriptome and metabolome analyses reveal novel insights into the seed germination of Michelia chapensis, an endangered species in China

文献类型： 外文期刊

第一作者： Zhou, Wuxian

作者： Zhou, Wuxian;Duan, Yuanyuan;Jiang, Xiaogang;Tan, Xuhui;Li, Qin;Wang, Hua;Zhang, Meide;Zhou, Wuxian;Duan, Yuanyuan;Jiang, Xiaogang;Tan, Xuhui;Li, Qin;Wang, Hua;Zhang, Meide;Zhang, Yajuan

作者机构：

关键词： Michelia chapensis; Seed germination; Plant hormone signal transduction; Starch and sucrose metabolism; Energy supply; Biosynthesis of secondary metabolites

期刊名称：PLANT SCIENCE （ 影响因子：5.2； 五年影响因子：5.7 ）

ISSN： 0168-9452

年卷期： 2023 年 328 卷

页码：

收录情况： SCI

摘要： Michelia chapensis Dandy, a well-known medicinal woody plant endemic to China, is endangered and seriously constricted by seed dormancy-induced low-regeneration in natural conditions. Cold stratification can effectively reduce seed dormancy and promote the seed germination of M. chapensis. However, the molecular events and systematic changes that occurred during seed germination in M. chapensis remain largely unknown. In this study, we carried out transcriptomic and metabolomic analyses to elucidate the potential molecular mechanisms un-derlying seed germination in M. chapensis under cold stratification. The results showed that the embryo cells became bigger and looser with increasing stratification time. Moreover, the endosperm appeared reduced due to the consumption of nutrients. Seventeen phytohormones were examined by the metabolome targeted for hor-mones. Compared with the ES (no stratification), the levels of indole-3-acetic acid (IAA) and gibberellin A3 (GA3) were increased in the MS (stratification for 45 days), while the abscisic acid (ABA) was downregulated in both MS and LS (stratification for 90 days). The transcriptome profiling identified 24975 differentially expressed genes (DEGs) in the seeds during germination. The seed germination of M. chapensis was mainly regulated by the biological pathways of plant hormone signal transduction, energy supply, secondary metabolite biosynthesis, photosynthesis-related metabolism, and transcriptional regulation. This study reveals the biological evidence of seed germination at the transcriptional level and provides a foundation for unraveling molecular mechanisms regulating the seed germination of M. chapensis.

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