Enhancing the recombinant protein productivity of Yarrowia lipolytica using in situ fibrous bed bioreactor

文献类型： 外文期刊

第一作者： Mou, Jin-Hua

作者： Mou, Jin-Hua;Wang, Zhen-Yao;Ong, Khai Lun;Qin, Zi-Hao;Lin, Carol Sze Ki;Ben Tahar, Imen;Fickers, Patrick;Li, Chong;Wang, Xiang

作者机构：

关键词： Free cell bioreactor; In situ fibrous bed bioreactor; Recombinant protein; Sugarcane bagasse; Yarrowia lipolytica

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）

ISSN： 0960-8524

年卷期： 2021 年 340 卷

页码：

收录情况： SCI

摘要： In this study, the ability of Yarrowia lipolytica to produce the recombinant lipase CalB from Candida antarctica, used as a model protein has been compared across different bioreactor processes using glycerol, a byproduct from the biodiesel industry as the main carbon source. Batch, pulsed fed-batch (PFB), and continuous fed-batch (CFB) strategies were first compared using classical stirred tank (STR) bioreactors in terms of biomass production, carbon source uptake, and lipase production. Additionally, an in situ fibrous bed bioreactor (isFBB) was developed using sugarcane bagasse as a cell immobilization support. The maximum lipase titer achieved using the isFBB culture mode was 38%, 33%, and 49% higher than those obtained using the batch, PFB, and CFB cultures, respectively. The lipase productivity in isFBB mode (142 U/mL/h) was 1.4-fold higher than that ob-tained using batch free cell cultures. These results highlight that isFBB is an efficient technology for the pro-duction of recombinant enzymes.

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