文献类型： 外文期刊

第一作者： Liu, Jie

作者： Liu, Jie;Shi, Fangyun;Wang, Chaoyue;Gao, Yang;Suo, Jingxia;Yu, Ying;Chen, Linlin;Zhang, Ning;Sun, Pei;Liu, Xianyong;Suo, Xun;Liu, Jie;Shi, Fangyun;Wang, Chaoyue;Gao, Yang;Suo, Jingxia;Yu, Ying;Chen, Linlin;Zhang, Ning;Sun, Pei;Liu, Xianyong;Suo, Xun;Zhang, Yuanyuan;Zhang, Yuanyuan;Tang, Xinming

作者机构：

关键词： Eimeria acervulina; Gametocyte; Stage-specific; Genetic cross

期刊名称：INTERNATIONAL JOURNAL FOR PARASITOLOGY （ 影响因子：4.0； 五年影响因子：3.8 ）

ISSN： 0020-7519

年卷期： 2023 年 53 卷 2 期

页码：

收录情况： SCI

摘要： Eimeria species are apicomplexan parasites with a direct life cycle consisting of a replicative phase involv-ing multiple rounds of asexual replication in the intestine or other organs including kidneys, liver, and gallbladder, depending on the species, followed by a sexual phase or gamogony involving the develop-ment and fertilization of gametes, an essential process for Eimeria transmission. Recent advances in the genetic manipulation of these parasites made it possible to conduct genetic crosses combined with genomic approaches to elucidate the genetic determinants of Eimeria development, virulence, drug resis-tance, and immune evasion. Here, we employed genetic techniques to generate two transgenic Eimeria acervulina lines, EaGAM56 and EaHAP2, each expressing two unique fluorescent proteins, with one con-trolled by a constitutive promotor for cross-efficiency analysis and the other by a male or female game-tocyte stage-specific promoter to observe sexual development. The expression of fluorescent proteins in the transgenic lines was analyzed in different developmental stages of the E. acervulina life cycle by immunoblotting and by examination of frozen sections using fluorescence microscopy. The effect of infective doses on cross-fertilization was further investigated by conducting several genetic crosses between the two transgenic lines at different doses and ratios. Two transgenic lines expressing constitu-tive and gametocyte-specific fluorescence proteins were generated and characterized. These transgenic parasites display synchronous development in chickens, comparable with that of the wild type. Genetic crosses between the two transgenic parasites showed that a high rate of oocysts co-expressing the two reporters could be achieved following inoculation with high doses of infective oocysts. We fur-ther showed that the proportion of co-transfected oocysts can be modulated by altering the ratio of the transgenic parental lines. Higher infective doses and similar numbers of functional gametocytes from the parents increase the rate of cross-fertilization. Our data highlight the usefulness of genetic manipulation and fluorescently-labeled transgenic gametocytes as tools to study Eimeria development and to elucidate the factors that modulate sexual development. This work sets the stage for the implementation of novel approaches to investigate other aspects of Eimeria pathogenesis, virulence, and drug susceptibility and resistance. (c) 2022 The Author(s). Published by Elsevier Ltd on behalf of Australian Society for Parasitology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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