文献类型： 外文期刊
第一作者： Yao, Yubo
作者： Yao, Yubo;Xue, Yongguo;Tang, Xiaofei;Cao, Dan;He, Wenjin;Luan, Xiaoyan;Liu, Qi;Zhu, Zifei;Liu, Xinlei;Yan, Jun
作者机构：
关键词： exogenous cytokinin; low phosphorus stress; nitrogen accumulation; nodule nitrogenase activity; soluble protein; free amino acid
期刊名称：AGRONOMY-BASEL （ 影响因子：3.4； 五年影响因子：3.8 ）
ISSN：
年卷期： 2025 年 15 卷 6 期
页码：
收录情况： SCI
摘要： Low phosphorus (P) stress impacts nitrogen (N) metabolism in soybeans. This study investigated the effects of exogenous cytokinin (Trans-Zeatin) on soybean N metabolism under low P stress by treating seeds with Trans-Zeatin and analyzing N accumulation, 15N abundance, nodule N fixation accumulation, nodule N fixation rate, nodule nitrogenase activity, soluble protein content, and free amino acid profiles. The results showed that exogenous cytokinin enhanced N accumulation in aboveground tissues, roots, and nodules, as well as nodule N fixation accumulation and fixation rate (from day 35 onward) under low P stress. Additionally, it promoted both acetylene reduction activity (ARA) and specific nitrogenase activity (SNA) in soybean nodules. By increasing the absorption of fertilizer-derived N, exogenous cytokinin alleviated the inhibitory effects of low P stress on the early growth and development of soybeans. Notably, under low P conditions, exogenous cytokinin significantly elevated the soluble protein content in nodules. However, the underlying mechanisms governing changes in free amino acid profiles require further investigation. This study provides a theoretical foundation for developing strategies to regulate soybean N metabolism under low P stress.
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