Multi-omics identification of a key glycosyl hydrolase gene FtGH1 involved in rutin hydrolysis in Tartary buckwheat (Fagopyrum tataricum)

文献类型： 外文期刊

第一作者： Lai, Dili

作者： Lai, Dili;Zhang, Kaixuan;He, Yuqi;Li, Wei;Shi, Yaliang;Gao, Yuanfen;Huang, Xu;He, Jiayue;Zhao, Hui;Lu, Xiang;Xiao, Yawen;Zhou, Meiliang;Lai, Dili;Cheng, Jianping;Ruan, Jingjun;Fan, Yu;Georgiev, Milen I.;Georgiev, Milen I.;Fernie, Alisdair R.;Fernie, Alisdair R.

作者机构：

关键词： Tartary buckwheat; Rutin; Hydrolysis; Re-sequencing; GWAS; Metabolome

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.8； 五年影响因子：13.2 ）

ISSN： 1467-7644

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Rutin, a flavonoid rich in buckwheat, is important for human health and plant resistance to external stresses. The hydrolysis of rutin to quercetin underlies the bitter taste of Tartary buckwheat. In order to identify rutin hydrolysis genes, a 200 genotypes mini-core Tartary buckwheat germplasm resource was re-sequenced with 30-fold coverage depth. By combining the content of the intermediate metabolites of rutin metabolism with genome resequencing data, metabolite genome-wide association analyses (GWAS) eventually identified a glycosyl hydrolase gene FtGH1, which could hydrolyse rutin to quercetin. This function was validated both in Tartary buckwheat overexpression hairy roots and in vitro enzyme activity assays. Mutation of the two key active sites, which were determined by molecular docking and experimentally verified via overexpression in hairy roots and transient expression in tobacco leaves, exhibited abnormal subcellular localization, suggesting functional changes. Sequence analysis revealed that mutation of the FtGH1 promoter in accessions of two haplotypes might be necessary for enzymatic activity. Co-expression analysis and GWAS revealed that FtbHLH165 not only repressed FtGH1 expression, but also increased seed length. This work reveals a potential mechanism behind rutin metabolism, which should provide both theoretical support in the study of flavonoid metabolism and in the molecular breeding of Tartary buckwheat.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序