Effect of cholesterol-loaded cyclodextrin treatment on boar sperm cryopreservation

文献类型： 外文期刊

第一作者： Zhang, Silong

作者： Zhang, Silong;Zhang, Hanbing;Liu, Kexiong;Xu, Xiaoling;Qin, Yusheng;Xiao, Linli;Liu, Yan;Bai, Jiahua;Zhang, Silong;Zhang, Hanbing;Zhou, Chunmei;Wu, Jianliang

作者机构：

关键词： Boar; Cholesterol; Cyclodextrin; Membrane Structure; Oxidative Damage; Sperm Total Motility

期刊名称：ANIMAL BIOSCIENCE （ 影响因子：2.5； 五年影响因子：2.8 ）

ISSN： 2765-0189

年卷期： 2024 年 37 卷 9 期

页码：

收录情况： SCI

摘要： Objective: This study investigated the efficacy of different concentrations of cholesterol- loaded cyclodextrin (CLC) on cryopreservation in boar sperm quality. Methods: In this study, we treated boar sperm with different concentrations of CLC before freezing and analyzed the sperm cholesterol concentration, plasma membrane, acrosome integrity rate and total motility rate before and after freeze-thawing. We also investigated the levels of reactive oxygen species (ROS), malondialdehyde (MDA), adenosine triphosphate (ATP), and structural- and oxidative-damage related proteins in all groups after thawing. Results: The results revealed that the cholesterol concentration of the CLC-treated groups was higher than that of the control group, both before freezing and after thawing (p<0.05). The plasma membrane integrity rate, acrosome integrity rate, and total motility rate of sperm were also enhanced after thawing in the CLC-treated group (all p<0.05). Moreover, ROS and MDA production and ATP loss were reduced in CLC-treated sperm during freezing and thawing (p<0.05). Finally, CLC pretreatment partially prevented the consumption of various proteins involved in metabolism including capping actin protein of muscle Z-line subunit beta (CAPZB), heat shock protein 90 alpha family class A member 1 (HSP90AA1) and phosphoglycerate mutase 2 (PGAM2) (p<0.05). Conclusion: The CLC treatment increased cholesterol concentration and decreased structural injury and oxidative damage during boar sperm freezing and thawing, improving the efficacy of sperm cryopreservation in boar.

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