Differential Resistance to Acetyl-CoA Carboxylase Inhibitors in Rice: Insights from Two Distinct Target-Site Mutations

文献类型： 外文期刊

第一作者： Zhou, Zhenzhen

作者： Zhou, Zhenzhen;Jiang, Qun;Qiu, Zeyu;Hou, Xiaodong;Yang, Xia;Yang, Yuwen;Hao, Tingting;Guo, Dongshu;Wang, Jinyan;Li, Yongfeng;Liu, Qing;Ling, Xitie;Zhang, Baolong;Zhou, Zhenzhen;Qiu, Zeyu;Hou, Xiaodong;Yang, Xia;Yang, Yuwen;Hao, Tingting;Guo, Dongshu;Wang, Jinyan;Li, Yongfeng;Liu, Qing;Ling, Xitie;Zhang, Baolong;Jiang, Qun;Zhang, Baolong;Liu, Qing

作者机构：

关键词： EMS mutagenesis; acetyl-CoA carboxylase; ACCase-inhibitingherbicide; herbicide resistance; rice

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 21 期

页码：

收录情况： SCI

摘要： Weeds present a significant challenge to agricultural productivity, and acetyl-CoA carboxylase (ACCase)-inhibiting herbicides have proven to be effective in managing weed populations in rice fields. To develop ACCase-inhibiting herbicide-resistant rice, we generated mutants of rice ACCase (OsACC) featuring Ile-1792-Leu or Gly-2107-Ser substitutions through ethyl methyl sulfonate (EMS) mutagenesis. The Ile-1792-Leu mutant displayed cross-resistance to aryloxyphenoxypropionate (APP) and phenylpyrazoline (DEN) herbicides, whereas the Gly-2107-Ser mutants primarily exhibited cross-resistance to APP herbicides with diminished resistance to the DEN herbicide. In vitro assays of the OsACC activity revealed an increase in resistance to haloxyfop and quizalofop, ranging from 4.84- to 29-fold in the mutants compared to that in wild-type. Structural modeling revealed that both mutations likely reduce the binding affinity between OsACC and ACCase inhibitors, thereby imparting resistance. This study offers insights into two target-site mutations, contributing to the breeding of herbicide-resistant rice and presenting alternative weed management strategies in rice cultivation.

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