Haploid-Phased Chromosomal Telomere-to-Telomere Genome Assembly of Medicinal Plant Uncaria rhynchophylla Dissects Genetic Controls on the Biosynthesis of Bioactive Alkaloids

文献类型： 外文期刊

第一作者： Hu, Tao

作者： Hu, Tao;Shangguan, Liyang;Zhao, Qingshi;Hang, Ye;Yan, Fulin;Lv, Qiuyu;Tang, Liu;Liu, Miao;Qiang, Wei;Zhang, Mingsheng;Duan, Lei;Wang, Xiaohong;Li, Xue;Yang, Ningxian;Wang, Xincun;Wang, Xuewen;Wang, Xuewen

作者机构：

关键词： alkaloid; biosynthesis; evolution; gene duplication; genome assembly; stress; Uncaria rhynchophylla

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：6.3； 五年影响因子：7.7 ）

ISSN： 0140-7791

年卷期： 2025 年 48 卷 3 期

页码：

收录情况： SCI

摘要： Natural indole alkaloids provide important medicinal resources and defences to environmental stresses. The Uncaria genus is a recorded traditional medicinal woody plant with high alkaloids. Genomic insights into alkaloid variation remain elusive. Here, we have dissected the haploid-resolved chromosomal T2T genome assembly of Uncaria rhynchophylla with a size of similar to 634 Mb and contig N50 of 27 Mb using PacBio HiFi long-reads plus Hi-C reads and anchored the contigs on 22 pairs of confirmed chromosomes. This genome contains 56% repeat sequences and similar to 29 000 protein-encoding genes. U. rhynchophylla diverged from a common ancestor shared with Coffea around 20 million years ago and contains expanded and contracted gene families associated with secondary metabolites and defences/resistance to stresses. We constructed the pathway and mined genes for rhynchophylline alkaloid biosynthesis. Fifty-three alkaloids in this pathway and eight differentially expressed genes are the keys to alkaloid accumulation. Elevated alkaloid levels are driven by high copy numbers of critical genes STRs and SGRs involved in strictosidine synthesis and hydrolysis as evidenced by phylogenetic, expression and RNA interference analyses. These results advance our genetic understanding and guide further breeding improvements, stress adaptation studies and pharmaceutical development.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序