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Biochemical characterization of a β-galactosidase from Lactiplantibacillus plantarum YLBGNL-S7 and utilization of its permeabilized whole-cell catalyst for in situ galactooligosaccharide synthesis during yogurt making

文献类型：外文期刊

第一作者： Li, Xin

作者： Li, Xin;Wang, Longlong;Guan, Bo;Xu, Chenxi;Hu, Youzhen;Han, Rui;Li, Xu;Ni, Yongqin;Li, Xin;Wang, Longlong;Guan, Bo;Xu, Chenxi;Hu, Youzhen;Han, Rui;Li, Xu;Ni, Yongqin;Li, Xin;Wang, Longlong;Guan, Bo;Xu, Chenxi;Hu, Youzhen;Han, Rui;Li, Xu;Ni, Yongqin;Zeng, Jun;Amirzada, Muhammad Imran

作者机构：

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （影响因子：6.6；五年影响因子：6.9 ）

ISSN： 0023-6438

年卷期： 2025 年 215 卷

页码：

收录情况： SCI

摘要： Recently, lactic acid bacteria (LAB)-derived (3-galactosidases have attracted much attention for their enzyme's high transglycosylation activity, GRAS status, and a tendency to form galactooligosaccharides (GOS) with a good prebiotic effect. However, their intracellular nature and the lack of a cost-effective method for the preparation of a food-grade catalyst hampers their application in the dairy industry. In this study, a (3-galactosidase LpLacLM from Lactiplantibacillus plantarum YLBGNL-S7 was characterized. In addition, the permeabilized whole cells of L. plantarum YLBGNL-S7, containing approximately 3.79 x 10-5 kat g-1 of (3-galactosidase activity, were prepared via lyophilization. The permeabilized whole cells could efficiently hydrolyze lactose and achieve maximum GOS yields of 31.71 g/ 100 g and 14.21 g/100 g at initial lactose concentrations of 30 g/100 mL and 5 g/100 mL, respectively. Moreover, the preincubation of reconstituted whole milk with 5.0 x 10- 8 kat mL- 1 of the permeabilized whole-cell catalyst at 50 degrees C for 3 h before the inoculation of traditional starters led to an 86.74% reduction in lactose and the production of 6.62 g L- 1 GOS in the yogurt. Through this study, we demonstrated a novel method to prepare permeabilized whole cells for LAB-derived (3-galactosidases and verified the feasibility of using this permeabilized whole-cell catalyst for in situ GOS synthesis during yogurt making.

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Biochemical characterization of a β-galactosidase from Lactiplantibacillus plantarum YLBGNL-S7 and utilization of its permeabilized whole-cell catalyst for in situ galactooligosaccharide synthesis during yogurt making

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