Identification of Plasma Membrane Aquaporin in Guard Cells of Vicia faba and Its Role in Stomatal Movement

文献类型： 外文期刊

第一作者： Huang, RF

作者： Huang, RF;Zhu, MJ;Kang, Y;Chen, J;Wang, XC

作者机构：

关键词： aquaporin;in situ hybridization;antibody of RD28;stomatal movement;vicia faba

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Water channels or aquaporins are the main pathways of water transport. Both the existence and function of aquaporins in the guard cells of Vicia faba L. were investigated both by using RD28 cDNA and RD28 antibody as probes, and by controlling stomatal movement as a parameter combined with antibody and inhibitor of aquaporins respectively. The results revealed that RD28 mRNA, encoding a plasma membrane aquaporin, expressed in the mesophyll cells and vascular tissues of V. faba, especially in guard cells. And the location of RD28-like proteins was mainly on plasma membrane of guard cells. The addition of 25 μmol/L HgCl_2, an aquaporin blocker, and antibody of RD28 as well, gready suppressed the stomatal opening or guardcell protoplast swelling induced by fusicoccin and light, and closing induced by abscisic acid. However, 5 mmol/L β-mercaptoethanol, a reverse reagent of aquaporin blocker, reversed the inhibitory effect of HgCl_2 pretreatment on stomatal opening (i.e. , HgCl_2 was removed after HgCl_2 pretreatment for 10 min). The results suggest that the aquaporins in V. faba are associated with stomatal movement.

分类号： Q94

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