Microsatellite Enrichment from AFLP Fragments by Magnetic Beads

文献类型: 外文期刊

第一作者: He, GH

作者: He, GH;Li, YR

作者机构:

关键词: microsatellite;AFLP;magnetic beads;peanut

期刊名称:ACTA BOTANICA SINICA ( 影响因子:0.599; )

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年卷期:

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收录情况: SCI

摘要: A simple and efficient method was presented for isolating microsatellite DNA markers from peanut (Arachis hypogaea L) genome. The genomic DNA was converted into pre-amplified AFLP fragments and hybridized with biotin-labeled SSR probes. Then the hybrid mixture was used to incubate with magnetic beads coated with streptavidin. After washing to remove the non-SSR fragments, the eluted single-strand DNA, which was cloned and sequenced, was largely enriched for microsatellites. Primers can then be designed according to the sequence flanking the repeat motifs and used for polymorphism analysis. The whole experiment can be completed within one week and can be employed as a reliable option for any molecular laboratory to develop SSR markers.

分类号: Q94

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