Homologous region 3 from Bombyx mori nucleopolyhedrovirus enhancing the transcriptional activity of heat shock cognate 70-4 promoter from Bombyx mori and Bombyx mandarina in vitro and in vivo
文献类型: 外文期刊
第一作者: Tang, SM
作者: Tang, SM;Zhao, QL;Yi, YZ;Zhang, ZF;Li, YR
作者机构:
关键词: heat shock cognate 70-4 protein gene;promoter;enhancer;BmN cell line;silk-worm
期刊名称:BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY ( 影响因子:2.043; 五年影响因子:2.017 )
ISSN: 0916-8451
年卷期: 2005 年 69 卷 5 期
页码:
收录情况: SCI
摘要: The heat shock cognate 70-4 protein gene promoter (HSC70-4p) from Bombyx mori (BmHSC70-4p) is an ideal candidate for the transgenic silkworm due to its high transcriptional activity, and the homologous region 3 from Bombyx mori nucleopolyhedrovirus (BmNPVhr3) functions as an enhancer for several promoters. Using luciferase as a reporter gene and transient expression system in vivo and in vitro, we found that BmNPVhr3 can significantly increase the transcriptional activity of BmHSC70-4p and HSC70-4p from Bombyx mandarina (BmandHSC70-4p). Moreover, the transcriptional activity of the combination of BmHSC70-4p and BmNPVhr3 changed with developmental stages and hormone titers. These results suggest that the combination of BmHSC70-4 promoter and BmNPVhr3 enhancer is more effective candidate for the transgene or stable cell expression system in Bombyx mori.
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