Identification of functional region of helicase gene promoter in Bombyx mori nuclear polyhedrosis virus

文献类型： 外文期刊

第一作者： Xiao, QL

作者： Xiao, QL;Zhang, ZF;Yi, YZ;He, JL;Wu, XF

作者机构：

关键词： Bombyx mori nuclear polyhedrosis virus;helicase gene promoter;transient expression;deletion analysis

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）

ISSN： 0582-9879

年卷期： 2002 年 34 卷 5 期

页码：

收录情况： SCI

摘要： DNA helicases are essential for replication of baculoviruses. It was found that the helicase gene promoter of Bombyx mori nuclear polyhedrosis virus, including 510 by upstream of ATG, had both early and late RNA initiation sites and could be recognized by cellular RNA polymerase. Transient expression assays in uninfected Sf-21 cells indicated that the helicase gene promoter could be classified as a delayed-curly gene promoter. Deletion analysis by PCR showed that the regulation region of its basic transcription was mainly within -510 to -410 by upstream of ATG. However, the basic activity was still detected with a deletion to -98 bp relative to ATG. In the presence of viral factors, deletion between -510 to -410 by relative to ATG did not significantly reduce the promoter activity compared to the full-length promoter (510 bp). The remarkable reduction in the promoter activity was observed with continuous deletions. It suggests, therefore, that c is-acting elements responsive to viral factors are mainly located within the range of -410 to -309 by upstream of ATG.

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