Analysis of simple sequence repeats markers derived from Phytophthora sojae expressed sequencetags

文献类型: 外文期刊

第一作者: Zhu, ZD

作者: Zhu, ZD;Huo, YL;Wang, XM;Huang, JB;Wu, XF

作者机构: Chinese Acad Agr Sci, Inst Crop Germplasm Resources, Beijing 100081, Peoples R China;Chinese Acad Agr Sci, Inst Crop Germplasm Resources, Beijing 100081, Peoples R China; Huazhong Agr Univ, Dept Plant Protect, Wuhan 430070, Peoples R China

关键词: expressed sequence tag;SSR marker;Phytophthora sojae;soybean

期刊名称:CHINESE SCIENCE BULLETIN ( 2016影响因子:1.649; 五年影响因子:1.738 )

ISSN: 1001-6538

年卷期: 2004 年 49 卷 19 期

页码:

收录情况: SCI

摘要: Five thousand and eight hundred publicly available expressed sequence tags (ESTs) of Phytophthora sojae were electronically searched and 415 simple sequence repeats (SSRs) were identified in 369 ESTs. The average density of SSRs was one SSR per 8.9 kb of EST sequence screened. The most frequent repeats were trinucleotide repeats (50.1%) and the least frequent were tetranucleotide repeats (8.2%). Forty primer pairs were designed and tested on 5 strains of P. sojae. Thirty-three primer pairs had successful PCR amplifications. Of the 33 functional primer pairs, 28 primer pairs produced characteristic SSR bands of the expected size, and 15 primer pairs (45.5%) detected polymorphism among 5 tested strains of P. sojae. Based on the polymorphisms detected with 20 EST-SSR markers, the 5 tested strains of P. sojae were clustered into 3 groups. In this study, the SSR markers of P. sojae were developed for the first time. These markers could be useful for identification, genetic variation study, and molecular mapping of P. sojae and its relative species.

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