High expression of foot-and-mouth disease virus structural protein VP1 in tobacco chloroplasts

文献类型： 外文期刊

第一作者： Li, Y

作者： Li, Y;Sun, M;Liu, JX;Yang, ZQ;Zhang, ZF;Shen, GF

作者机构：

关键词： foot-and-mouth disease virus (FMDV);VP1 protein;tobacco transformation;chloroplast

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2006 年 25 卷 4 期

页码：

收录情况： SCI

摘要： A tobacco chloroplast expression vector, pTRVP1, containing the foot-and-mouth disease virus (FMDV) VP1 gene and the selective marker aadA gene, was constructed and transferred to tobacco by biolistic method. Three resistant lines were obtained through spectinomycin selection, and each transgenic line was subjected to a second round of spectinomycin selection. PCR and PCR southern blot analysis revealed that the VP1 gene had integrated into the chloroplast genome. Western blot and quantification ELISA assays indicated that the VP1 gene was expressed in tobacco chloroplasts and accounted for 2-3% of total soluble protein. This suggested that plant chloroplasts were an efficient expression system for the potential production of recombinant antigens in plants.

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