Analysis of the molecular karyotype of Kengyilia grandiglumis on the Qinghai-Tibet Plateau via sequential FISH and GISH

文献类型： 外文期刊

第一作者： Liu, Ruijuan

作者： Liu, Ruijuan;Zhong, Yijun;Wu, Yue;Liu, Demei;Chen, Wenjie;Jiang, Liling;Zhong, Yijun;Wu, Yue

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关键词： Kengyilia grandiglumis; FISH; Translocation; Tandem repeat probes; TE probes

期刊名称：GENETIC RESOURCES AND CROP EVOLUTION

ISSN： 0925-9864

年卷期： 2025 年 72 卷 6 期

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收录情况： SCI

摘要： Kengyilia grandiglumis (Keng) J. L. Yang et al. is a perennial grass that developed in the sandy habitat of the Qinghai-Tibet Plateau. In this study, three-color FISH combined with sequential GISH was carried out on 80 individuals from 4 representative populations of K. grandiglumis. For the first time, two types of reciprocal translocations between the P and Y subgenomes have been identified in K. grandiglumis. These translocations were observed in populations QH1 and QH2, respectively, and the translocation chromosomes and frequency were different. The signals of the tandem repeat probe HvT01 were represented on chromosomes 3P, 6P, 7P, 1St, 2St, 5St, and 3Y, most of which were located on the subtelomeric regions. The simple sequence repeat AAG tended to be distributed on the pericentromeric regions and was detected on chromosomes 1 to 7Y, 2St, 3St, 4St, 5St, 6St and 6P. The chromosomal distribution of the TE probe S5 was concentrated on the telomere to intercalary regions of chromosomes 1P-7P, whereas in the Y and St subgenomes, the signal was scattered on the subtelomeric or pericentromeric regions of some chromosomes. On the basis of the diversity of the three probe signals within and among the 4 populations, we mapped the idiogram of FISH-banded chromosomes of K. grandiglumis. These results provide important theoretical guidance for the collection, protection, and evaluation of K. grandiglumis.

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