文献类型： 外文期刊
第一作者： Qi, Yanping
作者： Qi, Yanping;Zhang, Chunlei;Yuan, Shan;Xu, Xin;Sun, Shi;Zhang, Lixin;Liu, Luping;Wu, Tingting;Han, Tianfu;Wu, Cunxiang;Jiang, Bingjun
作者机构：
关键词： GmFT1a; liquid Y2H-Seq; protein-protein interaction
期刊名称：PROTEOMICS （ 影响因子：3.9； 五年影响因子：3.8 ）
ISSN： 1615-9853
年卷期： 2025 年 25 卷 13 期
页码：
收录情况： SCI
摘要： Highly laborious plating of cultures onto solid media is inevitable in conventional yeast two-hybrid systems. We have successfully developed Liquid Y2H-Seq, a method that replaces solid media with liquid media for culturing and displaces visual inspection of colonies with sequencing. Soybean (Glycine max) is a typical photoperiod-sensitive crop, meaning that a specific duration of light regime has a huge impact on soybean flowering and production. We obtained a lot of putative interactions for soybean flowering genes. Finally, these reports introduce a labor-reducing and time-saving method for identifying protein-protein interactions, based on simple modifications to a ubiquitous protocol in life science research.
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