Regulation of Glandular Size and Phytoalexin Biosynthesis by a Negative Feedback Loop in Cotton

文献类型： 外文期刊

第一作者： Wu, Wen-Kai

作者： Wu, Wen-Kai;Nie, Gui-Bin;Lin, Jia-Ling;Huang, Jia-Fa;Guo, Xiao-Xiang;Chen, Mei;Mao, Ying-Bo;Wang, Ling-Jian;Huang, Jin-Quan;Wu, Wen-Kai;Nie, Gui-Bin;Lin, Jia-Ling;Huang, Jia-Fa;Lin, Jia-Ling;Fang, Xin;Li, Yan;Li, Yan;Tao, Xiao-Yuan;Gao, Yiqun;Yang, Zuo-Ren;Yang, Zuo-Ren

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关键词： cotton; jasmonate; pigment gland; VQ domain

期刊名称：ADVANCED SCIENCE （ 影响因子：15.1； 五年影响因子：16.7 ）

ISSN：

年卷期： 2024 年

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收录情况： SCI

摘要： Plants have evolved diverse defense mechanisms encompassing physical and chemical barriers. Cotton pigment glands are known for containing various defense metabolites, but the precise regulation of gland size to modulate defense compound levels remains enigmatic. Here, it is discovered that the VQ domain-containing protein JAVL negatively regulates pigment gland size and the biosynthesis of defense compounds, while the MYC2-like transcription factor GoPGF has the opposite effect. Notably, GoPGF directly activates the expression of JAVL, whereas JAVL suppresses GoPGF transcription, establishing a negative feedback loop that maintains the expression homeostasis between GoPGF and JAVL. Furthermore, it is observed that JAVL negatively regulates jasmonate levels by inhibiting the expression of jasmonate biosynthetic genes and interacting with GoPGF to attenuate its activation effects, thereby maintaining homeostatic regulation of jasmonate levels. The increased expression ratio of GoPGF to JAVL leads to enlarged pigment glands and elevated jasmonates and defense compounds, enhancing insect and pathogen resistance in cotton. These findings unveil a new mechanism for regulating gland size and secondary metabolites biosynthesis, providing innovative strategies for strengthening plant defense. This study elucidates a negative feedback loop model involving a VQ-domain containing protein and a MYC2-like protein, whereby the ratio of their mRNA and protein contents regulates the size of pigment glands and the biosynthesis of secondary metabolites in cotton. These discoveries unveil a novel mechanism and present innovative strategies for enhancing plant defense, paving the avenue for the improvement of crops. image

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