Skin-specific transgenic overexpression of ovine beta-catenin in mice

文献类型： 外文期刊

第一作者： Wang, Jiankui

作者： Wang, Jiankui;Cui, Kai;Hua, Guoying;Han, Deping;Yang, Zu;Li, Tun;Yang, Xue;Zhang, Yuanyuan;Cai, Ganxian;Deng, Xiaotian;Deng, Xuemei;Cui, Kai

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关键词： beta-catenin; ovine; transgenic mice; overexpression; hair follicles

期刊名称：FRONTIERS IN GENETICS （ 影响因子：3.7； 五年影响因子：4.3 ）

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年卷期： 2023 年 13 卷

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收录情况： SCI

摘要： beta-catenin is a conserved molecule that plays an important role in hair follicle development. In this study, we generated skin-specific overexpression of ovine beta-catenin in transgenic mice by pronuclear microinjection. Results of polymerase chain reaction (PCR) testing and Southern blot showed that the ovine beta-catenin gene was successfully transferred into mice, and the exogenous beta-catenin gene was passed down from the first to sixth generations. Furthermore, real-time fluorescent quantitative PCR (qRT-PCR) and western blot analysis showed that beta-catenin mRNA was specifically expressed in the skin of transgenic mice. The analysis of F6 phenotypes showed that overexpression of beta-catenin could increase hair follicle density by prematurely promoting the catagen-to-anagen transition. The results showed that ovine beta-catenin could also promote hair follicle development in mice. We, therefore, demonstrate domestication traits in animals.

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