OsPRMT6a-mediated arginine methylation of OsJAZ1 regulates jasmonate signaling and spikelet development in rice

文献类型： 外文期刊

第一作者： Dong, Kun

作者： Dong, Kun;Wu, Fuqing;Li, Shuai;Zhang, Feng;Xing, Xinxin;Jin, Xin;Luo, Sheng;Feng, Miao;Chang, Yanqi;Zhang, Shuang;Zhang, Xin;Lei, Cailin;Ren, Yulong;Zhu, Shanshan;Guo, Xiuping;Wu, Chuanyin;Lin, Qibing;Cheng, Zhijun;Wan, Jianmin;Cheng, Siqi;Miao, Rong;You, Xiaoman;Wang, Peiran;Yang, Dong-Lei;Wan, Jianmin

作者机构：

关键词： protein arginine methylation; jasmonate signaling; negative feedback loop; spikelet development; adaptation to heat stress; rice

期刊名称：MOLECULAR PLANT （ 影响因子：17.1； 五年影响因子：21.4 ）

ISSN： 1674-2052

年卷期： 2024 年 17 卷 6 期

页码：

收录情况： SCI

摘要： Although both protein arginine methylation (PRMT) and jasmonate (JA) signaling are crucial for regulating plant development, the relationship between these processes in the control of spikelet development remains unclear. In this study, we used the CRISPR/Cas9 technology to generate two OsPRMT6a loss-offunction mutants that exhibit various abnormal spikelet structures. Interestingly, we found that OsPRMT6a can methylate arginine residues in JA signal repressors OsJAZ1 and OsJAZ7. We showed that arginine methylation of OsJAZ1 enhances the binding affinity of OsJAZ1 with the JA receptors OsCOI1a and OsCOI1b in the presence of JAs, thereby promoting the ubiquitination of OsJAZ1 by the SCFOsCOI1a/OsCOI1b complex and degradation via the 26S proteasome. This process ultimately releases OsMYC2, a core transcriptional regulator in the JA signaling pathway, to activate or repress JA-responsive genes, thereby maintaining normal plant (spikelet) development. However, in the osprmt6a-1 mutant, reduced arginine methylation of OsJAZ1 impaires the interaction between OsJAZ1 and OsCOI1a/ OsCOI1b in the presence of JAs. As a result, OsJAZ1 proteins become more stable, repressing JA responses, thus causing the formation of abnormal spikelet structures. Moreover, we discovered that JA signaling reduces the OsPRMT6a mRNA level in an OsMYC2-dependent manner, thereby establishing a negative feedback loop to balance JA signaling. We further found that OsPRMT6a-mediated arginine methylation of OsJAZ1 likely serves as a switch to tune JA signaling to maintain normal spikelet development under harsh environmental conditions such as high temperatures. Collectively, our study establishes a direct molecular link between arginine methylation and JA signaling in rice.

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