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Identification of Reliable Reference Genes for the Expression of Hydrangea macrophylla 'Bailmer' and 'Duro' Sepal Color

文献类型：外文期刊

第一作者： Zhang, Gaitian

作者： Zhang, Gaitian;Yuan, Suxia;Qi, Hui;Chu, Zhiyun;Liu, Chun

作者机构：

关键词： sepals; flower color change; reference gene

期刊名称：HORTICULTURAE （影响因子：2.923；五年影响因子：3.582 ）

ISSN：

年卷期： 2022 年 8 卷 9 期

页码：

收录情况： SCI

摘要： Hydrangea spp. is renowned for its variety of color changes in its developmental stage and before and after aluminum treatment. We analyzed gene expression in hydrangeas sepals to study the causes of color change. The accuracy of quantitative RT-qPCR analysis depends on the reliability of reference genes. We selected reference genes for hydrangea of varying cultivars, at different developmental stages, and in aluminum treatment groups. We chose 'Bailmer' and 'Duro' as subject species. We selected eight candidate genes, all of which were ranked by geNorm, NormFinder, BestKeeper, and RefFinder. CCR, NHX1, and LODX were used to verify the exactitude of reference genes. According to the ranking result of RefFinder, the top-ranked reference genes in each group were different; the top four candidate reference genes in each group mostly included EF1-beta, RPL34, GADPH, and RPL10. EF1-beta and RPL34 ranked top in the 'all materials' group, and their expression trends, obtained from the analysis of CCR, NHX1, and LODX, were consistent. From the results, we gather that EF1-beta and RPL34 can be used as reference genes to quantify target gene expression. In this study, we screened for reference genes in hydrangeas to provide a technical basis for hydrangea sepal formation and transformation for further experiments.

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