Trilobatin Acts as a Marker Metabolite Involved in Flavonoid Accumulation Regulated by CsWRKY28-MYC2 with Trypsin Activation in Cucumber (Cucumis sativus)

文献类型： 外文期刊

第一作者： Chen, Enyan

作者： Chen, Enyan;Jia, Jingyu;Sun, Jiaju;Chen, Xinxin;Li, Xin;Wang, Jie;Jia, Jingyu;Li, Xin;Li, Xin

作者机构：

关键词： CsWRKY28; transcriptomics; trilobatin; trypsin; widely targeted metabolomics

期刊名称：PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：1.2； 五年影响因子：1.3 ）

ISSN： 0031-9457

年卷期： 2024 年 93 卷 11 期

页码：

收录情况： SCI

摘要： During post-harvest storage of Cucumis sativus fruit, the application of trypsin treatment could increase fl avonoid compound levels and reduce oxidative damage. To investigate the mechanism of trypsin-induced fl avonoid biosynthesis in C. sativus , we conducted a combined analysis of transcriptomics and widely targeted metabolomics. One hundred and seventy- fi ve signi fi cantly different metabolites were obtained from metabolomics data. The kyoto encyclopedia of genes and genomes (KEGG) functional enrichment results indicated that these metabolites were mainly involved in the phenylpropanoid biosynthesis pathway. By combining the results of the weighted gene co-expression network analysis (WGCNA) with the 130 upregulated phenylpropanoid metabolites, 22 signi fi cantly upregulated phenylpropanoid metabolites were identi fi ed. Trilobatin was identi fi ed as the most prominent metabolite through cluster analysis and variable importance in projection (VIP) analysis. High performance liquid chromatography (HPLC) experiments con fi rmed that trilobatin was the key metabolite induced by trypsin. The transcriptomic results showed that 1068 genes in the brown module of WGCNA were highly positively correlated with fl avonoid biosynthesis. The gene set enrichment analysis (GSEA) identi fi ed leading edges in 4 key KEGG pathways. Finally, combined with WGCNA and GSEA analysis results, 35 core genes were obtained. The co-expression network of transcriptomics and metabolomics suggested that CsWRKY28 and CsMYC2 regulated the biosynthesis of trilobatin. The quantitative real-time polymerase chain reaction (RT-qPCR) and dual luciferase experiments confi rmed the activation effect of CsWRKY28 on CsMYC2 and downstream target genes. This study revealed the key transcription factors involved in the trypsin-controlled biosynthesis of trilobatin in C. sativus and provided a new theoretical basis for elucidating the molecular mechanism of trypsin preservation.

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