Toxic effects and transcriptional responses in zebrafish liver cells following perfluorooctanoic acid exposure

文献类型： 外文期刊

第一作者： Wu, Dong-Lei

作者： Wu, Dong-Lei;Cheng, Lin;Rao, Qin-Xiong;Wang, Xian-Li;Zhang, Qi-Cai;Yao, Chun-Xia;Yao, Xia;Chen, Shan-Shan;Liu, Xing;Song, Xei;Zhou, Jia-Xin;Song, Wei-Guo;Wu, Dong-Lei;Cheng, Lin;Rao, Qin-Xiong;Wang, Xian-Li;Zhang, Qi-Cai;Yao, Chun-Xia;Yao, Xia;Chen, Shan-Shan;Liu, Xing;Song, Xei;Zhou, Jia-Xin;Song, Wei-Guo

作者机构：

关键词： PFOA; Zebrafish liver cells; Toxicity; RNA-Seq; Transcriptome

期刊名称：AQUATIC TOXICOLOGY （ 影响因子：4.5； 五年影响因子：5.2 ）

ISSN： 0166-445X

年卷期： 2022 年 253 卷

页码：

收录情况： SCI

摘要： As a typical type of persistent organic pollutant, perfluorooctanoic acid (PFOA) is pervasive in the environment. Multiple studies have found that PFOA has hepatotoxicity, but the mechanism remains poorly understood. In this study, the toxic effects of different concentrations of PFOA on zebrafish liver cells were systematically assessed by recording cell survival, ultrastructural observations, and transcriptome analyses. The results showed that the inhibition of cell viability and the massive accumulation of autophagic vacuoles were observed at 400 mu M PFOA, while transcriptomic changes occurred with treatments of 1 and 400 mu M PFOA. The transcription levels of 1055 (977 up-and 78 down-regulated genes) and 520 (446 up-and 74 down-regulated genes) genes were significantly changed after treatment with 1 and 400 mu M PFOA, respectively. Based on Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis, significant expression changes were observed in autophagy, tight junction, signal transduction, immune system, endocrine system, and metabolism-related pathways, indicating that such processes were greatly affected by PFOA exposure. The findings of this study will provide a scientific basis for the toxic effects and potential toxic mechanisms of PFOA on zebrafish, and provide information for ecological risk assessments.

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