Dissection of mRNA ac4C acetylation modifications in AC and Nr fruits: insights into the regulation of fruit ripening by ethylene

文献类型： 外文期刊

第一作者： Ma, Lili

作者： Ma, Lili;Zheng, Yanyan;Bai, Chunmei;Wang, Qing;Zuo, Jinhua;Zhou, Zhongjing;Deng, Zhiping;Tan, Jinjuan;Fu, Anzhen

作者机构：

关键词： ac(4)C modification; Tomato; Ethylene; Fruit ripening; mRNA

期刊名称：MOLECULAR HORTICULTURE （ 影响因子：8.1； 五年影响因子：11.0 ）

ISSN： 2730-9401

年卷期： 2024 年 4 卷 1 期

页码：

收录情况： SCI

摘要： N-4-acetylcytidine (ac(4)C) modification of mRNA has been shown to be present in plant RNAs, but its regulatory function in plant remains largely unexplored. In this study, we investigated the differentially expressed mRNAs, lncRNAs and acetylation modifications of mRNAs in tomato fruits from both genotypes. By comparing wild-type (AC) tomato and the ethylene receptor-mutant (Nr) tomato from mature green (MG) to six days after the breaker (Br6) stage, we identified differences in numerous key genes related to fruit ripening and observed the corresponding lncRNAs positively regulated the target genes expression. At the post-transcriptional level, the acetylation level decreased and increased in AC and Nr tomatoes from MG to Br6 stage, respectively. The integrated analysis of RNA-seq and ac(4)C-seq data revealed the potential positive role of acetylation modification in regulating gene expression. Furthermore, we found differential acetylation modifications of certain transcripts (ACO, ETR, ERF, PG, CesA, beta-Gal, GAD, AMY, and SUS) in AC and Nr fruits which may explain the differences in ethylene production, fruit texture, and flavor during their ripening processes. The present study provides new insights into the molecular mechanisms by which acetylation modification differentially regulates the ripening process of wild-type and mutant tomato fruits deficient in ethylene signaling.

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